Feb 14, 2012

FOXM1 expression correlates with tumor invasion and a poor prognosis of colorectal cancer

Acta histochemica
Xiaoyuan ChuXiao-Bei Mo

Abstract

FOXM1, a member of the Forkhead Box (Fox) family of transcription factors, plays a critical role in tumor development and metastasis. The aim of this study was to elucidate its role in colorectal cancer (CRC), particularly prognosis and metastasis. Semi-quantitative RT-PCR and Western blot assays were used to measure the expression levels of FOXM1 mRNA and protein in 15 CRC and adjacent normal mucosa tissues. Immunohistochemical assay was performed to detect FOXM1 protein expression in 112 CRC tissues and further determine its clinicopathological and prognostic significance. RNA interference (RNAi) was used to knockdown endogenous FOXM1 expression in CRC cell lines and to analyze the effects of FOXM1 knockdown on migration and invasion of CRC cells. The relative expression levels of FOXM1 mRNA and protein were significantly higher in CRC tissues than in adjacent normal mucosa tissues (P<0.01). In addition, the immunostaining of FOXM1 protein was stronger in CRC tissues than in adjacent normal mucosa tissues. By statistical analysis, we showed that high FOXM1 expression was closely correlated with the presence of lymph node metastasis, incidence of liver metastasis, and advanced TNM stage. Moreover, the cumulative 5-year surviva...Continue Reading

  • References22
  • Citations33

Citations

Mentioned in this Paper

Cell Cycle Progression
Gene Expression Regulation, Neoplastic
Transfection
Immunohistochemistry
RNA, Small Interfering
Cell Motility
Western Blotting
Transcription, Genetic
GAPDH gene
Liver Carcinoma

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