FoxO1 and FoxA1/2 form a complex on DNA and cooperate to open chromatin at insulin-regulated genes

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Daniel SchillLisa Ann Cirillo

Abstract

We have previously shown that cooperative, interdependent binding by the pioneer factors FoxO1 and FoxA1/2 is required for recruitment of RNA polymerase II and H3K27 acetylation to the promoters of insulin-regulated genes. However, the underlying mechanisms are unknown. In this study, we demonstrate that, in HepG2 cells, FoxO1 and FoxA2 form a complex on DNA that is disrupted by insulin treatment. Insulin-mediated phosphorylation of FoxO1 and FoxA2 does not impair their cooperative binding to mononucleosome particles assembled from the IGFBP1 promoter, indicating that direct disruption of complex formation by phosphorylation is not responsible for the loss of interdependent FoxO1:FoxA1/2 binding following insulin treatment. Since FoxO1 and FoxA1/2 binding is required for the establishment and maintenance of transcriptionally active chromatin at insulin-regulated genes, we hypothesized that cooperative FoxO1 and FoxA1/2 binding dictates the chromatin remodeling events required for the initial activation of these genes. In support of this idea, we demonstrate that FoxO1 and FoxA2 cooperatively open linker histone compacted chromatin templates containing the IGFBP1 promoter. Taken together, these results provide a mechanism for ho...Continue Reading

References

Jun 8, 1999·The Journal of Biological Chemistry·E D TangK L Guan
Jun 8, 1999·The Journal of Biological Chemistry·G RenaP Cohen
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·W H BiggsK C Arden
Sep 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·N NasrinM C Alexander-Bridges
Sep 26, 2001·The Journal of Biological Chemistry·D ChayaK S Zaret
Jan 18, 2003·Developmental Cell·Jun NakaeDomenico Accili
Jan 31, 2003·The Journal of Biological Chemistry·Beth T Vander KooiRichard M O'Brien
Sep 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christian WolfrumMarkus Stoffel
Jun 17, 2005·Nature·Catherine S LeeKlaus H Kaestner
Aug 24, 2005·Oncogene·Yan-Nan LiuJi Hshiung Chen
Aug 16, 2006·Cellular and Molecular Life Sciences : CMLS·J R Friedman, K H Kaestner
Jul 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jian XuStephen T Smale
Oct 10, 2007·The Journal of Biological Chemistry·Mitsutoki Hatta, Lisa Ann Cirillo
Jan 26, 2008·Circulation Research·Heather J Evans-AndersonKatherine E Yutzey
Jul 9, 2009·In Vitro Cellular & Developmental Biology. Animal·Keisuke Munekata, Kazuichi Sakamoto
Nov 10, 2009·The Journal of Biological Chemistry·Sarah Kohler, Lisa Ann Cirillo
Jan 4, 2017·PloS One·Roya DerwishLaura L Gillespie

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Citations

May 7, 2020·Cancer Metastasis Reviews·Yannasittha Jiramongkol, Eric W-F Lam
Feb 13, 2021·Nucleic Acids Research·Jun LiYongheng Chen
Apr 24, 2020·Biochimica Et Biophysica Acta. Reviews on Cancer·Bing GaoJianping Guo

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