FoxO1 expression in osteoblasts modulates bone formation through resistance to oxidative stress in mice

Biochemical and Biophysical Research Communications
Yixin ZhangYingying Wu

Abstract

Accumulation of reactive oxygen species (ROS) induced by oxidative stress (OS) affects cell survival, cell function and even results in cell death. As a major transcription factor of forkhead O (FoxOs) family, FoxO1 orchestrates multiple osteoblastic biological processes, thus regulating osteoblast physiology and bone metabolism. However, the outcome of osteoblast behaviors varies under different physiological and pathological conditions. Also, the underlying impact of FoxO1 on oxidative stress and further on bone metabolism still remains unclear. In this study, using osteoblast-specific FoxO1 knockout (FoxO1OB-/-) mice, we investigated the potential roles of FoxO1 on bone formation and osteoblast bioactivity under physiological condition. We show herein that FoxO1-knockout decreased bone volume and bone formation rate in FoxO1OB-/- mice, which might be related to the decreased osteoblasts number. We also found that FoxO1-knockout increased apoptosis-related caspase-3 activity of osteoblasts, and inhibited the expression of osteogenic phenotypic markers (i.e. Runx2, Osx, ALP and OPN), leading to reduced osteoblasts differentiation. The alterations of bone formation and osteoblasts bioactivity were further testified to be linked...Continue Reading

Citations

Dec 26, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Xinnan WuQing Ji
Nov 30, 2018·PloS One·Laura De-UgarteNatalia Garcia-Giralt
Feb 23, 2021·Cell Proliferation·Jiachao GuoJingfan Shao
Jun 13, 2020·Stem Cell Reports·Shoko OnoderaShinsuke Ohba

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