Apr 25, 2020

Identification of simplified microbial communities that inhibit Clostridioides difficile infection through dilution/extinction

BioRxiv : the Preprint Server for Biology
J. M. AuchtungRobert A Britton

Abstract

The gastrointestinal microbiome plays an important role in limiting susceptibility to infection with Clostridioides difficile. To better understand the ecology of bacteria important for C. difficile colonization resistance, we developed an experimental platform to simplify complex communities of fecal bacteria through dilution and rapidly screen for their ability to inhibit C. difficile. We simplified complex communities from six fecal donors and found that 17% of simplified communities inhibited C. difficile growth when initially isolated and when re-cultured from frozen stocks. Composition varied between simplified communities based upon fecal donor used for dilution; complexity ranged from 19-67 OTUs. One simplified community could be further simplified through dilution and retain the ability to inhibit C. difficile. We tested efficacy of seven simplified communities in a humanized microbiota mouse model and found that four communities were able to significantly reduce the severity of the initial C. difficile infection and limit susceptibility to disease relapse. Analysis of fecal microbiomes from treated mice demonstrated that simplified communities accelerated recovery of endogenous bacteria and led to stable engraftment o...Continue Reading

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Mentioned in this Paper

Neurospora crassa
In Vivo
Patterns
Phosphothreonine
Cellular Process
Infection by Cryptococcus Neoformans
Saccharomyces cerevisiae allergenic extract
Post-Translational Protein Processing
PNKD
Protein Phosphorylation

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