FPR1 gene silencing suppresses cardiomyocyte apoptosis and ventricular remodeling in rats with ischemia/reperfusion injury through the inhibition of MAPK signaling pathway

Experimental Cell Research
Qing-Ling ZhouGuo-Wei Meng

Abstract

Ischemia/reperfusion (I/R) injury, one of the leading health problems in the world, is defined as a cause of cardiomyocytes death. In the present study, we investigate the role of formyl peptide receptor 1 (FPR1) in cardiomyocyte apoptosis and ventricular remodeling of I/R injury rats and the underlying mechanism involving mitogen-activated protein kinase (MAPK) signaling pathway. The important differentially expressed genes (DEGs) in I/R injury were screened out and downstream pathways affected by DEGs were predicted. We grouped 90 rats into sham, I/R, NC siRNA, FRP1 siRNA, empty vector, and FRP1 vector groups and established a model of I/R injury in rats. CVF value, myocardial infarct areas and positive expression rate of FPR1 and MAPK were detected. Levels of FPR1 and MAPK pathway-related genes were determined by RT-qPCR and western blot analysis. MTT assay was performed to evaluate cell proliferation and flow cytometry to evaluate cell cycle progression and apoptosis. GSE19804 and GSE27262 were screened from Gene Expression Omnibus database. FPR1 was higher in patients with I/R injury and activate the MAPK signaling pathway. FRP1 gene silencing decreased CVF value, infarct area, apoptotic index, positive expression rates of...Continue Reading

Citations

May 12, 2020·Oncoimmunology·Erika VacchelliGuido Kroemer
Jul 6, 2019·Cardiology Research and Practice·Nan-Bo LiuXiao-Kang Li
Oct 23, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nicole J EdwardsBenjamin Levi
Jan 26, 2021·Oncoimmunology·Zsofia SztupinszkiGuido Kroemer
Dec 22, 2020·Oncoimmunology·Adriana PetrazzuoloGuido Kroemer
Mar 19, 2020·Cellular Immunology·Hrishikesh S KulkarniAndrew E Gelman
Mar 5, 2021·Journal of Cardiovascular Pharmacology·Hefeng WangDan Li

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