Fractal dimension analysis reveals skeletal muscle disorganization in mdx mice

Biochemical and Biophysical Research Communications
Sarah Santiloni CuryRobson Francisco Carvalho

Abstract

Duchenne Muscular Dystrophy (DMD) is characterized by muscle extracellular matrix disorganization due to the increased collagen deposition leading to fibrosis that significantly exacerbates disease progression. Fractal dimension analysis is a method that quantifies tissue/cellular disorganization and characterizes complex structures. The first objective of the present study was use fractal analysis to evaluate extracellular matrix disorganization in mdx mice soleus muscle. Next, we mimic a hyper-proliferation of fibrogenic cells by co-culturing NIH3T3 fibroblasts and C2C12 myoblasts to test whether fibroblasts induce disorganization in myoblast arrangement. Here, we show mdx presented high skeletal muscle disorganization as revealed by fractal analysis. Similarly, this method revealed that myoblasts co-cultured with fibroblast also presented cellular arrangement disorganization. We also reanalyzed skeletal muscle microarrays transcriptomic data from mdx and DMD patients that revealed transcripts related to extracellular matrix organization. This analysis also identified Osteoglycin, which was validated as a potential regulator of ECM organization in mdx dystrophic muscles. Our results demonstrate that fractal dimension is usefu...Continue Reading

Citations

Aug 29, 2020·Journal of Cardiovascular Pharmacology and Therapeutics·Francine DuchatschSandra L Amaral
Sep 10, 2020·Environmental Science and Pollution Research International·Raíssa De Oliveira MantovaniFrancis Lopes Pacagnelli
Jan 5, 2021·Journal of Cardiovascular Pharmacology·Danyelle S MiottoSandra L Amaral
Jul 3, 2021·International Journal of Molecular Sciences·Lara Caetano RochaAdriano Polican Ciena

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