PMID: 6971123Dec 4, 1980Paper

Fractionation and partial characterization of alpha-1-protease isoinhibitors of horse

Biochimica Et Biophysica Acta
A Pellegrini, R von Fellenberg

Abstract

The principal alpha-1-protease inhibitor of horse was fractionated by classical methods and analysed with a modified fibrinogen-agarose gel electrophoretic method of high sensitivity and resolving power. Starting with an electrophoretically homogeneous inhibitor in unfractionated serum, two isoinhibitor bands became apparent after fractionation with (NH4)2SO4 and DEAE-cellulose DE-52 ion-exchange chromatography. The isoinhibitors differed in electrophoretic migration and in the elution pattern from Sephadex G-100 gel filtration, but possessed identical antigenic determinants and enzyme specificity. The slower migrating isoinhibitor with an apparent molecular weight of 90 000 could be highly purified. In contrast the faster moving isoinhibitor (molecular weight 65 000) could not be completely freed from a contaminating alpha-2-protease inhibitor. The formation of the two isoinhibitors is discussed considering conformational changes analogous to phenomena observed with alpha-2-macroglobulin, or dimer formation in combination with altered conformations. The isoinhibitors described here are new additions to the different heterogeneities which exist in alpha-1-protease inhibitors in horse. They also supplement the different heteroge...Continue Reading

References

Aug 1, 1979·The Biochemical Journal·A J BarrettC A Sayers
Jan 1, 1979·Animal Blood Groups and Biochemical Genetics·R K JunejaK Sandberg
Aug 22, 1978·Biochemistry·W R AbramsG Weinbaum
Nov 16, 1978·The New England Journal of Medicine·J O Morse
Jan 1, 1979·Animal Blood Groups and Biochemical Genetics·A G Matthews
Dec 1, 1975·Analytical Biochemistry·C E Stauffer
Apr 1, 1973·The Biochemical Journal·P M Starkey, A J Barrett
Jul 1, 1974·Analytical Biochemistry·S C MarchP Cuatrecasas
Mar 23, 1971·Biochimica Et Biophysica Acta·F Kueppers
Jul 1, 1970·Genetics·M Braend
Sep 1, 1967·European Journal of Biochemistry·J Labouesse, M Gervais
Nov 30, 1967·Biochemical and Biophysical Research Communications·T Chase, E Shaw

❮ Previous
Next ❯

Citations

Jan 1, 1983·The International Journal of Biochemistry·A PellegriniR von Fellenberg
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A PellegriniR von Fellenberg
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M Eguchi, Y Yamamoto
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S D Patterson
Aug 1, 1985·Biological Chemistry Hoppe-Seyler·R von FellenbergA Pellegrini
Mar 7, 1996·Biochimica Et Biophysica Acta·Z GalzieA Salahuddin
Jan 1, 1983·The International Journal of Biochemistry·A PellegriniR von Fellenberg
Jan 1, 1985·The International Journal of Biochemistry·A PellegriniR von Fellenberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The International Journal of Biochemistry
A PellegriniR von Fellenberg
Comparative Biochemistry and Physiology. B, Comparative Biochemistry
S D PattersonD C Shaw
Comparative Biochemistry and Physiology. B, Comparative Biochemistry
W F DivenJ Chambers
© 2021 Meta ULC. All rights reserved