PMID: 109132Jun 1, 1979

Fractionation of antibodies to L-cell colony-stimulating factor by affinity chromatography.

R K ShadduckL Higgins


Purified L-cell colony-stimulating factor (CSF) was coupled to cyanogen-bromide-activated Sepharose and used to selectively fractionate antibodies to this factor. With the use of a simplified two-step washing and elution technique, there was 50%--70% binding of the anti-CSF, with recovery of 60%--100% of the bound material. Both the native antiserum and purified anti-CSF fractions were inhibitory to murine granulocyte-macrophage colony formation. The purified antibodies contained only IgG and were reduced in protein concentration to 0.1% of the serum IgG values. These fractions should prove useful tools for the study of granulocyte and macrophage differentiation.

Related Concepts

Antibody Specificity
Binding Sites, Antibody
Chromatography, Affinity
Chromatography, Agarose
Protein Inducer MGI
Dose-Response Relationship, Immunologic
Chemical Fractionation
Immunodiffusion Measurement

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