PMID: 8586068Oct 1, 1995Paper

Fractionation of carrier ampholytes in multicompartment electrolyzers with isoelectric membranes

Electrophoresis
A Bossi, P G Righetti

Abstract

Multicompartment electrolyzers with isoelectric membranes can be successfully utilized for the preparation of carrier ampholytes of narrow pH ranges from commercially available wider pH intervals. An example is given on the preparation of a 0.6 pH unit (pH 6.7-7.3) range from a standard pH 6-8 interval. A 10% Ampholine solution is focused in an electrolyzer equipped with the following isoelectric membranes: pI 6.0, 6.7, 7.3 and 8.0. The narrow pH 6.7-7.3 cut can be efficiently utilized for base-line separation and quantitation of hemoglobin A from its glycated form, Hb A1c. This analysis is important for the screening and follow up of diabetic patients. The advantage of multicompartment electrolyzers are: (i) precision in the preparation of narrow pH cuts, due to the presence of membranes with defined pI values; (ii) ability to perform in both small- and large-scale operations and (iii) absence of contaminants leaching from granulated supports, as typical of some previous techniques.

References

Jun 18, 1975·Journal of Chromatography·E GianazzaP G Righetti
Jan 1, 1978·American Journal of Hematology·L BeccariaP G Righetti
Mar 1, 1977·Analytical Biochemistry·W I OtavskyJ W Drysdale
Sep 25, 1974·Journal of Chromatography·P G Righetti, J W Drysdale
Jun 15, 1973·Annals of the New York Academy of Sciences·J S Fawcett
Sep 1, 1970·Analytical Biochemistry·V Macko, H Stegemann
Sep 1, 1982·American Journal of Hematology·G CossuP G Righetti
Sep 1, 1983·Journal of Biochemical and Biophysical Methods·C Gelfi, P G Righetti
Dec 1, 1981·Journal of Biochemical and Biophysical Methods·P G Righetti, S Hjertén

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Citations

May 14, 2004·Journal of Chromatography. a·Evan Shave, Gyula Vigh
Dec 25, 2002·Journal of Chromatography. a·David OgleGyula Vigh
Mar 19, 2003·Journal of Chromatography. a·Evan Shave, Gyula Vigh
Jul 14, 1998·Electrophoresis·P G Righetti, A Bossi
Dec 15, 2005·Analytical Chemistry·Huanchun CuiCornelius F Ivory

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