Fractionation of honey carbohydrates using pressurized liquid extraction with activated charcoal

Journal of Agricultural and Food Chemistry
Ana I Ruiz-MatuteM Luz Sanz

Abstract

This article describes the development of a new procedure that combines the use of activated charcoal and pressurized liquid extraction (PLE) to obtain enriched fractions of di- and trisaccharides from honey. Honey was adsorbed onto activated charcoal and packed into a PLE extraction cell. Optimum results were obtained at 10 MPa and 40 degrees C using two consecutive PLE cycles: first, 1:99 (v/v) ethanol/water for 5 min and second, 50:50 (v/v) ethanol/water for 10 min. Di- and trisaccharide fractions were enriched after PLE treatment, accounting for 73% and 8% of total carbohydrates, respectively. This procedure was also compared with other methodologies reported in the literature for the fractionation of honey carbohydrates (yeast treatment and extraction from activated charcoal). While the removal of monosaccharides was more efficient with yeast treatment, recovery of di- and trisaccharides was higher when either the PLE or the activated charcoal treatment was used. PLE was found to be the faster technique; it also required less solvent volume and minimized handling of the sample.

References

Jan 23, 2004·Journal of Chromatography. a·J F CotteM F Grenier-Loustalot
Apr 14, 2005·Journal of Agricultural and Food Chemistry·María Luz SanzRobert A Rastall
Aug 7, 2007·Journal of Agricultural and Food Chemistry·A I Ruiz-MatuteM L Sanz

❮ Previous
Next ❯

Citations

Apr 5, 2013·Journal of Agricultural and Food Chemistry·Sonia Rodríguez-SánchezAna C Soria
Sep 24, 2015·International Journal of Molecular Sciences·Maria Hayes, Brijesh K Tiwari
Jan 29, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yu LiuYinsheng Qiu
Dec 6, 2011·Journal of Chromatography. a·Lourdes Ramos
Sep 29, 2015·Journal of Chromatography. a·Fernando MontañésRahau Shirazi
Jul 27, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jia GuanShao-Ping Li
Jun 22, 2021·Critical Reviews in Biotechnology·Christian K O DzuvorFrancis Kubi
Jun 14, 2021·Food Chemistry·Jorge Ederson G SantanaRaimundo Nonato Pereira Teixeira

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.