Fractionation of particulate glycogen and bound enzymes using high-performance liquid chromatography

Analytical Biochemistry
A Vardanis

Abstract

During a study aimed at the specificity of binding of phosphorylase and glycogen synthase to glycogen particles we investigated the separation of these particles with the technique of high-performance liquid chromatography using several media. The technique allowed relatively rapid fractionations with little, if any, loss of enzyme activity. The basis of some separations was molecular weight of the particle. Of the molecular exclusion media used, Superose 6 had an exclusion limit large enough to accommodate most glycogens but excluded mouse liver and bovine liver glycogen particles. Toyopearl HW-75 F was a medium that could accommodate liver glycogen and gave us reasonably well-defined separation of the particles that carried phosphorylase and glycogen synthase. Separations on ion-exchange columns, that were obviously based on the overall charge of proteins associated with the glycogen particles, were not very effective in terms of allowing the separation of different types of particle. Of all the media used the hydrophobic interaction column was best in terms of separating what appeared to be distinct populations of particles that had definite preferences in terms of binding phosphorylase and glycogen synthase. As expected, th...Continue Reading

References

May 1, 1971·Archives of Biochemistry and Biophysics·S DiMauroL P Rowland
Dec 1, 1970·Clinica Chimica Acta; International Journal of Clinical Chemistry·F Huijing
Mar 1, 1969·Archives of Biochemistry and Biophysics·A Vardanis
Jun 1, 1961·The Journal of Biophysical and Biochemical Cytology·D J LUCK

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Citations

Aug 29, 2000·Journal of Experimental Marine Biology and Ecology·K B BrokordtH E Guderley
Mar 26, 2013·Proteome Science·David StapletonGlendon Parker
Sep 5, 2007·Biochemical and Biophysical Research Communications·Glendon J ParkerDavid Stapleton
Apr 15, 2010·Proteomics·David StapletonGlendon Parker
Sep 28, 2012·American Journal of Physiology. Cell Physiology·Robyn M MurphyDavid I Stapleton
Apr 22, 2016·American Journal of Physiology. Cell Physiology·Hongyang XuRobyn M Murphy

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