Fractionation of plant aminoacyl-tRNA synthetases on tRNA-Sepharose columns.

Biochimica Et Biophysica Acta
H Jakubowski, J Pawelkiewicz

Abstract

It has been shown that tRNA-Sepharose, a chromatographic adsorbent containing unfractionated tRNA bound to a Sepharose matrix, is a useful, group-specific adsorbent for fractionation of the plant aminoacyl-tRNA synthetases. Conditions are described in which Val-, Trp-, Phe-, Leu- and Ile-tRNA synthetases from yellow lupin seeds can be separated from each other on the tRNA-Sepharose columns. Factors affecting affinity chromatography on the t-RNA-Sepharose columns are discussed. The affinity chromatography procedure for the purification of lupin Ser-tRNA synthetase to homogenity is described.

References

Jan 1, 1975·Nucleic Acids Research·H Wierzbicka Pawelkiewicz
Jun 22, 1972·Biochimica Et Biophysica Acta·S Bartkowiak, J Pawelkiewicz
Mar 27, 1972·European Journal of Biochemistry·H BeikirchF Cramer
Dec 4, 1972·European Journal of Biochemistry·M Robert-Gero, J P Waller
Mar 1, 1973·Canadian Journal of Biochemistry·P I Forrester, R L Hancock
Apr 2, 1973·European Journal of Biochemistry·F von der Haar
Oct 23, 1974·Biochemical and Biophysical Research Communications·C M Joyce, J R Knowles

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