Dec 22, 1982

Fractionation of protein components of plasma membranes from the electric organ of Torpedo marmorata

Biochimica Et Biophysica Acta
L ElfmanP L Jørgensen


A procedure has been developed for the separation of intrinsic proteins of plasma membranes from the electric organ of Torpedo marmorata. (Na+ + K+)-ATPase, nicotinic acetylcholine receptor and acetylcholinesterase remained active after solubilization with the nonionic detergent dodecyl octaethylene glycol monoether (C12E8). These components could be separated by ion exchange chromatography on DEAE-Sephadex A-25. Fractions enriched in ouabain-sensitive K+-phosphatase or (Na+ + K+)-ATPase activity showed two bands in sodium dodecyl sulphate polyacrylamide gel electrophoresis corresponding to the alpha- and beta-subunits. The (Na+ + K+)-ATPase was shown to have immunological determinants in common with a 93 kDa polypeptide which copurified with the nicotinic acetylcholine receptor, also after solubilization in Triton X-100 and chromatography on Naja naja siamensis alpha-toxin-Sepharose columns. The data suggest that the alpha-subunit of (Na+ + K+)-ATPase associates with the acetylcholine receptor in the membranes of the electric organ.

Mentioned in this Paper

Tissue Membrane
Phosphoric Monoester Hydrolases
Adenosine Triphosphatases
Naja siamensis
Torpedo marmorata
Cholinergic Receptors
Phosphoric Monoester Hydrolase Activity
Chemical Fractionation

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