PMID: 6971Jun 1, 1976Paper

Fractionation of serum transcobalamins on charged cellulose filters

Proceedings of the Society for Experimental Biology and Medicine
J SelhubN Grossowicz

Abstract

A simple and rapid fractionation procedure of the three transcobalamins, TCI, TCII, and TCII, of human serum was achieved by filtration through a stack of charged cellulose filters composed of one cellulose-nitrate and three DEAE-cellulose (DE-81) disks. A reaction mixture containing microliter amounts of serum was incubated with excess of 57Co B12 of high specific activity, diluted with 0.1 M sodium borate buffer (pH 8.5), and passed through the filter stack by applying vacuum. Under these conditions TCII is selectively and quantitatively adsorbed to the cellulose-nitrate filter while both TCI and TCIII adsorb to the DE-81 filters. In the second step TCIII is selectively desorbed from the latter filters by a 0.05 M monopotassium phosphate solution of pH 4.6. Using sera of different distribution of transcobalamins the data obtained were comparable to those determined by the more laborious methods employing DE-52 column chromatography combined with procedures to remove TCII.

Citations

Jan 1, 1983·European Journal of Clinical Pharmacology·M GranatM Rachmilewitz
Feb 1, 1990·The American Journal of Medicine·Y MoladA Weinberger
Nov 30, 1981·Biochemical and Biophysical Research Communications·J A BegleyC A Hall
Jan 1, 1982·Critical Reviews in Clinical Laboratory Sciences·F Fernandes-Costa, J Metz
Dec 1, 1977·Scandinavian Journal of Clinical and Laboratory Investigation·E Nexø, J Andersen
Nov 1, 1977·Scandinavian Journal of Haematology·B RachmilewitzM Rachmilewitz
Oct 13, 1999·Journal of Inherited Metabolic Disease·H BibiD S Rosenblatt

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