Fractionation of whey proteins with high-capacity superparamagnetic ion-exchangers

Journal of Biotechnology
Anders Heebøll-NielsenOwen R T Thomas

Abstract

In this study we describe the design, preparation and testing of superparamagnetic anion-exchangers, and their use together with cation-exchangers in the fractionation of bovine whey proteins as a model study for high-gradient magnetic fishing. Adsorbents prepared by attachment of trimethyl amine to particles activated in sequential reactions with allyl bromide and N-bromosuccinimide yielded a maximum bovine serum albumin binding capacity of 156 mg g(-1) combined with a dissociation constant of 0.60 microM, whereas ion-exchangers created by linking polyethylene imine through superficial aldehydes bound up to 337 mg g(-1) with a dissociation constant of 0.042 microM. The latter anion-exchanger was selected for studies of whey protein fractionation. In these, crude bovine whey was treated with a superparamagnetic cation-exchanger to adsorb basic protein species, and the supernatant arising from this treatment was then contacted with the anion-exchanger. For both adsorbent classes of ion-exchanger, desorption selectivity was subsequently studied by sequentially increasing the concentration of NaCl in the elution buffer. In the initial cation-exchange step quantitative removal of lactoferrin (LF) and lactoperoxidase (LPO) was achie...Continue Reading

References

Mar 15, 1982·Analytical Biochemistry·G Vanecek, F E Regnier
Jul 4, 1998·Journal of Chromatography. a·S J Gerberding, C H Byers
Mar 9, 2002·Journal of Chromatography. a·Bo Xue, Yan Sun
Jun 7, 2003·Biotechnology Progress·Anders Heebøll-NielsenOwen R T Thomas
Jul 29, 2004·Biotechnology and Bioengineering·Anders Heebøll-NielsenOwen R T Thomas

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Citations

Feb 24, 2006·Applied Microbiology and Biotechnology·Matthias FranzrebOwen R T Thomas
Jun 9, 2005·Current Opinion in Microbiology·Karl Schügerl, Jürgen Hubbuch
May 1, 2009·Biotechnology and Bioengineering·Jagan M Billakanti, Conan J Fee
Jan 25, 2011·Biotechnology Journal·Kim Ekelund OttowTimothy J Hobley
Mar 23, 2011·Biotechnology Letters·Mayyada M H El-Sayed, Howard A Chase
Oct 16, 2019·Frontiers in Bioengineering and Biotechnology·Sebastian P SchwamingerSonja Berensmeier
Jan 23, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Layla PanahipourReinhard Gruber

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