Fractionation Protocol for the Isolation of Polypeptides from Plant Biomass

Journal of Natural Products
Per ClaesonLars Bohlin

Abstract

A fractionation protocol for the isolation of a highly purified polypeptide fraction from plant biomass is described. The procedure dereplicates ubiquitous substance classes known to interfere with bioassays often used in natural product-based drug discovery programs. The protocol involves pre-extraction with dichloromethane, extraction with ethanol (50%), removal of tannins with polyamide, removal of low-molecular-weight components with size-exclusion chromatography over Sephadex G-10, and final removal of salts and polysaccharides with solid-phase extraction using reversed-phase cartridges. The method has been applied to the aerial parts of Viola arvensis, resulting in the isolation of a peptide fraction that on further separation yielded a novel 29-residue macrocyclic polypeptide named varv peptide A, cyclo(-TCVGGTCNTPGCSCSWPVCTRNGLPVCGE-).

References

Sep 6, 1996·Science·W Roush
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·D R BergeyC A Ryan
Feb 1, 1996·Journal of Natural Products·E Haslam

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Citations

Apr 7, 2006·Journal of Industrial Microbiology & Biotechnology·David J Newman, Russell T Hill
Aug 29, 2001·Phytochemistry·A M BroussalisP Claeson
Aug 30, 2003·Phytochemistry·Erika SvangårdPer Claeson
Aug 11, 2000·Toxicon : Official Journal of the International Society on Toxinology·D J CraikC Waine
Oct 16, 2001·Toxicon : Official Journal of the International Society on Toxinology·D J Craik
Mar 15, 2002·Trends in Biochemical Sciences·Manuela Trabi, David J Craik
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·C JenningsM Anderson
Oct 2, 2009·Cellular and Molecular Life Sciences : CMLS·David J CraikNorelle L Daly
Apr 4, 2012·Expert Opinion on Drug Discovery·David J CraikMasa Cemazar
Dec 27, 2015·Analytical Biochemistry·Mohsen FarhadpourAlireza Ghassempour
Mar 12, 2010·Toxicon : Official Journal of the International Society on Toxinology·David J Craik
May 9, 2009·Trends in Plant Science·David J Craik
Jun 26, 2007·Peptides·Patrícia B PelegriniOctávio L Franco
Jun 6, 2013·Journal of Peptide Science : an Official Publication of the European Peptide Society·David J Craik
Apr 20, 2006·Journal of Ethnopharmacology·Sékou BahHarald T Johansen
Jan 17, 2007·Toxicon : Official Journal of the International Society on Toxinology·Christian W GruberDavid J Craik
Nov 3, 2009·Drug Discovery Today·Sónia Troeira Henriques, David J Craik
Jan 14, 2017·Biochemistry·Sónia Troeira Henriques, David J Craik
Mar 31, 2015·Chembiochem : a European Journal of Chemical Biology·Teshome L AboyeUlf Göransson
Mar 21, 2007·Molecular BioSystems·David J Craik, Norelle L Daly
May 23, 2009·Organic & Biomolecular Chemistry·Manuela TrabiDavid J Craik
Aug 12, 2010·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Lars BohlinAnders Backlund
Jun 22, 2010·Biopolymers·Michelle L ColgraveDavid J Craik
Jun 8, 2001·Anti-cancer Drugs·S JohanssonP Claeson
Apr 28, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Claudio Andrés ÁlvarezFanny Guzmán
Sep 17, 2002·Journal of Biomolecular Screening·Petra LindholmLars Bohlin
Sep 10, 2003·The Journal of Biological Chemistry·Ulf Göransson, David J Craik

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