PMID: 6165988Jan 1, 1981Paper

Fracture-label:O cytochemistry of freeze-fracture faces in the erythrocyte membrane

Proceedings of the National Academy of Sciences of the United States of America
P Pinto Da SilvaN Dwyer

Abstract

A method--"fracture label"--is described for the cytochemical labeling of the membrane faces produced by freeze-fracture. Human erythrocytes embedded in a crosslinked matrix are frozen, fractured in liquid nitrogen, thawed, labeled, and cut into thin sections. Electron microscope observation of the fracture faces shows preferential partition of concanavalin A binding sites with the inner half of the membrane. This signifies that, during freeze-fracture, binding sites are dragged from the outer surface across the outer ("exoplasmic") half of the membrane and retained on the protoplasmic fracture face (face P). The fracture process results in exposure of new anionic sites on face P. Fracture-label can be applied to the cytochemical characterization of the cellular components exposed by freeze-fracture of isolated cells and tissues.

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Citations

Jul 1, 1996·Histochemistry and Cell Biology·M R Torrisi, P Mancini
Apr 7, 2007·Histochemistry and Cell Biology·Wiebke SchlörmannWalter Richter
Oct 1, 1988·Journal of Ultrastructure and Molecular Structure Research·M Carmo-Fonseca
Apr 5, 2007·Nature Protocols·Nicholas J Severs
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M R Torrisi, P P Da Silva
Feb 1, 1985·The Journal of Cell Biology·A P Aguas, P Pinto da Silva
Feb 1, 1986·The Journal of Cell Biology·K A Fisher, K C Yanagimoto
Jan 1, 1984·The Journal of Cell Biology·M R Torrisi, P Pinto da Silva
Aug 1, 1984·The Journal of Cell Biology·A P Aguas, P Pinto da Silva
Sep 1, 1984·The Journal of Cell Biology·P Pinto da Silva, F W Kan
Mar 7, 1983·Brain Research·R DermietzelD Schünke
Jul 7, 1985·Journal of Theoretical Biology·M J KellW Goolsby
Mar 11, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T TakizawaJ M Robinson
Jan 1, 1991·Cell Motility and the Cytoskeleton·J A UrsittiR J Bloch
Nov 1, 1989·Journal of Electron Microscopy Technique·N J Severs
Nov 1, 1989·Journal of Electron Microscopy Technique·J E Rash, F D Giddings
Aug 1, 1995·Microscopy Research and Technique·R R Shivers, L K McVicar
Jul 29, 2008·Memórias do Instituto Oswaldo Cruz·Wanderley de Souza
Jun 1, 1986·Journal of Microscopy·H P Ting-BeallJ D Robertson
Apr 1, 1982·Journal of Microscopy·K A Fisher

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