Fragaria chiloensis cryptic virus: A New Strawberry Virus Found in Fragaria chiloensis Plants from Chile

Plant Disease
I E Tzanetakis, R R Martin

Abstract

Molecular characterization of Fragaria chiloensis latent virus (FClLV) (3) was based on shotgun cloning of double-stranded RNA (dsRNA) obtained from a Chilean Fragaria chiloensis plant. While the majority of the clones acquired belonged to FClLV, several had similarities with the RNA polymerases of viruses of the family Partitiviridae. A region of more than 1 kb of the putative virus (GenBank Accession No. DQ093961) was acquired by a combination of shotgun cloning and reverse transcription-polymerase chain reaction (RT-PCR) (3) and was compared against sequences found in GenBank. The Alphacryptovirus Beet cryptic virus 3 (Genbank Accession No. S63913) and the RNA-dependent RNA polymerase encoded using dsRNA isolated from Pyrus pyrifolia (Genbank Accession No. BAA34783) produced the most significant alignments with approximately 40% amino acid sequence identity and 60% amino acid similarity with the coding region of the sequenced portion of the virus. Detection primers F (5'AAGTCCGTGAGCACTGCCAT3') and R (5'TGAATACAAGTAACGGGAATTGA3') that amplify a 152-bp fragment of the putative virus were developed and used for RT-PCR detection of the virus as described (4) in 20 F. chiloensis plants obtained from the National Clonal Germplasm ...Continue Reading

Citations

Jul 7, 2020·EFSA Journal·UNKNOWN EFSA Panel on Plant Health (PLH)Luisa Rubino
Apr 1, 2006·Plant Disease·Robert R Martin, Ioannis E Tzanetakis

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