Obestatin is a twenty three amino acid peptide produced in the stomach by post translational modification of the preproghrelin gene. Since its discovery in 2005, many studies have shown that obestatin reduces feed intake and gain in body weight in rodents. Studies from our laboratory have shown the N-terminal thirteen residues mimic obestatin the best and residues 6-18 reduce epididymal fat significantly in adult male mice. In this study we have tried to increase the efficacy of these fragments. As an initial step, we have substituted G(8) with alpha-aminoisobutyricacid(Aib,U) and F(5) with cyclohexylalanine(Cha) in the N-terminal peptide to obtain two modified peptides and modified the middle fragment (residues 6-18) by substituting both the glycine residues at position 3 and 8 with alpha-aminoisobutyricacid(U). The rationale being, unusual amino acids could protect the peptides from immediate degradation and Aib would also induce secondary structure in these unstructured peptides. The N-terminal fragment with the G(8)U substitution fared the best. It reduced food intake, gain in body weight, levels of cholesterol and triglycerides in the blood, epididymal and perirenal fat in adult male mice similar to that of obestatin. The ...Continue Reading
Reference values for the determination of GOT, GPT, and alkaline phosphatase in serum with optimal standard methods (author's transl)
The purification and properties of the alpha-glycerophosphate-oxidizing enzyme of Streptococcus faecalis 10C1
Lack of interaction between peripheral injection of CCK and obestatin in the regulation of gastric satiety signaling in rodents
Neither intravenous nor intracerebroventricular administration of obestatin affects the secretion of GH, PRL, TSH and ACTH in rats
Little or no ability of obestatin to interact with ghrelin or modify motility in the rat gastrointestinal tract
Comment on "Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake".
Direct and indirect effects of obestatin peptides on food intake and the regulation of glucose homeostasis and insulin secretion in mice
Decreased obestatin in plasma in metabolically obese, normal-weight men with normal glucose tolerance
Conformational manifold of alpha-aminoisobutyric acid (Aib) containing alanine-based tripeptides in aqueous solution explored by vibrational spectroscopy, electronic circular dichroism spectroscopy, and molecular dynamics simulations
Effects of single intranasal administration of obestatin fragments on the body weight and feeding and drinking behaviors
Chronic treatment with a stable obestatin analog significantly alters plasma triglyceride levels but fails to influence food intake; fluid intake; body weight; or body composition in rats
Studies on the influence of CCK-8 on the ability of obestatin to reduce food intake, gain in body weight and related lipid parameters
Peripheral administration of TAT-obestatin can influence the expression of liporegulatory genes but fails to affect food intake in mice
Obestatin as a key regulator of metabolism and cardiovascular function with emerging therapeutic potential for diabetes
Treatment of lean and diet-induced obesity (DIO) mice with a novel stable obestatin analogue alters plasma metabolite levels as detected by untargeted LC-MS metabolomics
Capsaicin And Genistein Override The Action Of Obestatin To Decrease Lipid Accumulation In 3T3-L1 Cells
Obestatin and Rosiglitazone Differentially Modulate Lipid Metabolism Through Peroxisome Proliferator-activated Receptor-γ (PPARγ) in Pre-adipose and Mature 3T3-L1 Cells.
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