Fragment ion patchwork quantification for measuring site-specific acetylation degrees

Analytical Chemistry
Rasha ElBashirAndreas Schlosser

Abstract

We introduce fragment ion patchwork quantification as a new mass spectrometry-based approach for the highly accurate quantification of site-specific acetylation degrees. This method combines (13)C1-acetyl derivatization on the protein level, proteolysis by low-specificity proteases and quantification on the fragment ion level. Acetylation degrees are determined from the isotope patterns of acetylated b and y ions. We show that this approach allows to determine site-specific acetylation degrees of all lysine residues for all core histones of Trypanosoma brucei. In addition, we demonstrate how this approach can be used to identify substrate sites of histone acetyltransferases.

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Citations

Jun 19, 2018·Nucleic Acids Research·Juan-José VasquezT Nicolai Siegel
Mar 23, 2017·BMC Bioinformatics·Willy Vincent BienvenutCarmela Giglione
Mar 22, 2020·Nature Communications·Amelie J KrausT Nicolai Siegel
Mar 30, 2021·Molecular & Cellular Proteomics : MCP·Francesca ZappacostaRoland S Annan

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African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.