Fragmentation of amyloid fibrils occurs in preferential positions depending on the environmental conditions

The Journal of Physical Chemistry. B
Lucrèce NicoudMassimo Morbidelli

Abstract

Understanding the mechanism of amyloid fibril breakage is of fundamental importance in various research fields including biomedicine and bionanotechnology. The aim of this work is to clarify the impact of temperature and agitation speed on the fibril breakage rate constant, which depends both on the fibril length as well as on the position of fragmentation along the fibril longitudinal axis. In particular, we intend to discriminate between three fibril fragmentation mechanisms: erosion (i.e., breakage occurs preferentially at the ends of the fibril), random (i.e., breakage occurs with the same likelihood at any position), or central (i.e., breakage occurs preferentially at the center of the fibril). To do so, we compare the time evolution of the fibril length distribution followed with atomic force microscopy with simulations from a kinetic model based on population balance equations (PBE). In this frame, we investigate the breakage mechanism of insulin fibrils, which turns out to be affected by the operative conditions employed. Moreover, we compare our findings with literature data obtained with β-lactoglobulin and β2-microglobulin. It is observed that high temperature drives the breakage toward an erosion mechanism, while a ...Continue Reading

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Aug 15, 2014·The Journal of Physical Chemistry. B·Lucrèce NicoudMassimo Morbidelli

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Citations

Feb 18, 2016·Scientific Reports·Srivastav RanganathanRanjith Padinhateeri
Mar 24, 2016·The Journal of Physical Chemistry. B·Lucrèce NicoudMassimo Morbidelli
Apr 2, 2016·Analytical Biochemistry·Paolo ArosioSara Linse
Sep 5, 2015·Physical Chemistry Chemical Physics : PCCP·Lucrèce NicoudMassimo Morbidelli
May 29, 2016·Advances in Colloid and Interface Science·S LazzariM Morbidelli
Feb 28, 2019·Soft Matter·Mohammadhosein RazbinAli Akbar Moosavi-Movahedi
Oct 2, 2020·Advanced Science·Ibrahim JavedPu Chun Ke
Dec 23, 2016·Biophysics Reviews·Ran ZhaoDamien Hall
Feb 14, 2021·International Journal of Molecular Sciences·Mantas ZiaunysVytautas Smirnovas
Jan 26, 2017·Biophysical Journal·Alireza AbdolvahabiBryan F Shaw
May 8, 2021·The Journal of Physical Chemistry. B·Aditi SharmaAndreas S Bommarius
Apr 12, 2019·Chemical Reviews·Ioana M Ilie, Amedeo Caflisch
Oct 15, 2020·ACS Chemical Neuroscience·Jennifer JosephRanjith Padinhateeri
Jan 27, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Xiuping HaoXiaoyan Dong
Feb 13, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Stefano LazzariKlavs F Jensen
Jun 28, 2019·The Journal of Physical Chemistry. B·Carlos PerezGhanim Ullah

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