Fragmentation of colicins A and E1 by cell surface proteases.

Journal of Bacteriology
R N Brey

Abstract

Interaction of either colicin A or E1 with the surface of Escherichia coli cells resulted in extensive cleavage of the colicins into many peptide fragments in the molecular weight range of 10,000 to 30,000 released into the supernatants of colicin-cell mixtures. The protease inhibitor P-aminobenzamidine inhibited the cleavage of colicin A and enhanced colicin killing activity, suggesting that proteolysis is not required for the killing action of colicin. Fragments derived from the supernatants of the mixtures were inactive against sensitive cells. Proteolytic activity against both colicins was localized primarily in the outer membrane fraction of the cell envelope. At least two distinct protease activities appear to be present. Examination of the patterns of cleavage and inactivation of the colicins by a series of resistant mutants indicates that specific colicin receptors play no essential role in colicin proteolysis. In addition, evidence is presented that adsorption of colicin to specific receptors is a reversible process.

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M J Weiss, S E Luria
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H Tokuda, J Konisky
Jan 1, 1979·Journal of Bacteriology·C H MacGregorJ E Blech
Feb 10, 1976·Biochemistry·C Lau, F M Richards
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K Schaller, M Nomura
Oct 1, 1967·Journal of Bacteriology·R Nagel de Zwaig, S E Luria
Jan 1, 1967·Annual Review of Microbiology·M Nomura
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S P LeytusW F Mangel

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Citations

Jan 1, 1984·The Journal of Membrane Biology·V L DavidsonF S Cohen
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Eric CascalesDanièle Cavard
Mar 21, 1983·Biochimica Et Biophysica Acta·W A CramerY Uratani

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