Fragmentation of phosphopeptides by atmospheric pressure MALDI and ESI/Ion trap mass spectrometry

Journal of the American Society for Mass Spectrometry
Susanne C MoyerAmina S Woods

Abstract

An investigation of phosphate loss from phosphopeptide ions was conducted, using both atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI) and electrospray ionization (ESI) coupled to an ion trap mass spectrometer (ITMS). These experiments were carried out on a number of phosphorylated peptides in order to investigate gas phase dephosphorylation patterns associated with phosphoserine, phosphothreonine, and phosphotyrosine residues. In particular, we explored the fragmentation patterns of phosphotyrosine containing peptides, which experience a loss of 98 Da under collision induced dissociation (CID) conditions in the ITMS. The loss of 98 Da is unexpected for phosphotyrosine, given the structure of its side chain. The fragmentation of phosphoserine and phosphothreonine containing peptides was also investigated. While phosphoserine and phosphothreonine residues undergo a loss of 98 Da under CID conditions regardless of peptide amino acid composition, phosphate loss from phosphotyrosine residues seems to be dependent on the presence of arginine or lysine residues in the peptide sequence.

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Citations

Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Wells W WuRong-Fong Shen
Feb 8, 2005·Journal of the American Society for Mass Spectrometry·Katherine A KellersbergerDaniele Fabris
Jan 31, 2006·Journal of the American Society for Mass Spectrometry·Scott D HantonJerry Zweigenbaum
Nov 26, 2008·Journal of the American Society for Mass Spectrometry·Lisa A VasicekJennifer S Brodbelt
May 1, 2009·Journal of the American Society for Mass Spectrometry·Yi ZhangJarrod A Marto
Oct 13, 2009·Journal of the American Society for Mass Spectrometry·Tae-Young Kim, James P Reilly
May 5, 2005·Regulatory Peptides·Jin-Tang DuHiroshi Nakanishi
Jun 5, 2003·Journal of the American Society for Mass Spectrometry·Susanne C MoyerRobert J Cotter
Jun 27, 2008·Journal of Proteome Research·Amina S WoodsShelley N Jackson
Dec 4, 2012·Biochimica Et Biophysica Acta·Kai-Wei HsuehChao-Hsiung Lin
Oct 23, 2015·The Journal of Physical Chemistry. B·Michele L StoverDavid A Dixon
Sep 15, 2011·Journal of Mass Spectrometry : JMS·Marko Rožman
Feb 5, 2011·Mass Spectrometry Reviews·Amanda M PalumboGavin E Reid
Nov 10, 2009·Proteomics·Christof RampitschNatalia V Bykova
Mar 24, 2016·Rapid Communications in Mass Spectrometry : RCM·Michael KrappmannThomas Letzel
Jun 9, 2009·Journal of Mass Spectrometry : JMS·Paul J BoersemaAlbert J R Heck
Jun 9, 2010·Journal of Mass Spectrometry : JMS·Arti T Navare, Facundo M Fernández
Nov 6, 2009·Rapid Communications in Mass Spectrometry : RCM·Soojin ParkHan Bin Oh
Jul 19, 2002·Journal of Mass Spectrometry : JMS
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Jan 13, 2006·Rapid Communications in Mass Spectrometry : RCM·Jérôme LemoinePhilippe Dugourd
Nov 11, 2018·Journal of the American Society for Mass Spectrometry·Inae JangHan Bin Oh

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