Fragmentation of re-formation of mitotic Golgi apparatus detected by a centrifugal method

Experimental Cell Research
K Nagata-KunoS Minakami

Abstract

The fragmentation/re-formation process of the Golgi apparatus during mitosis was studied by flotation centrifugation in a stepwise sucrose density gradient. The mitotic Golgi fraction was obtained from Chinese hamster ovary cells synchronized with thymidine and nocodazole. The Golgi apparatus detected by a marker enzyme, galactosyltransferase, was separated into two peaks by the flotation centrifugation. The amount of the Golgi recovered at the lower density peak was less in the mitotic cells than in the interphase cells. The separation profile of the mitotic Golgi returned to that of the interphase Golgi by further incubation of the mitotic cells. The re-formation of the fragmented Golgi was inhibited by nocodazole and vinblastine, but not by actinomycin D and cycloheximide.

References

Aug 1, 1989·The Journal of Cell Biology·J M LucocqG Warren
Apr 1, 1987·The Journal of Cell Biology·J M LucocqG Warren
Jul 1, 1985·Experimental Cell Research·J Thyberg, S Moskalewski

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Citations

Apr 4, 1998·Microscopy Research and Technique·J Thyberg, S Moskalewski
Apr 4, 1998·Molecular Biology of the Cell·S A Jesch, A D Linstedt
Oct 21, 2003·Molecular Biology of the Cell·Sapna Puri, Adam D Linstedt
Feb 2, 1999·Experimental Cell Research·J Thyberg, S Moskalewski
Mar 1, 1993·Biochimica Et Biophysica Acta·T ShigemuraN Hamasaki

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