Fragmented hyaluronan has no alarmin function assessed in arthritis synovial fibroblast and chondrocyte cultures

Innate Immunity
Mia OlssonHelena Erlandsson Harris

Abstract

Hyaluronan (HA) is a large polymer and an important component of the extracellular matrix. During homeostasis, high molecular mass HA is the predominant form, but upon inflammation, degradation products of HA accumulate. These HA fragments (HA-fs) have been reported to possess pro-inflammatory activities and thus act as alarmins, notifying immune cells of danger via TLR4 and CD44. HA is found in large quantities in synovial joint fluid. In order to reveal a potential role of HA-fs in arthritis pathogenesis, the in vitro effects of HA of various molecular masses (from 1680 kDa to oligosaccharide HA) on synovial fibroblasts and chondrocytes from rheumatoid arthritis patients, and on peripheral blood mononuclear cells from healthy donors, were investigated. TLR4 and CD44 surface expression was confirmed by immunocytochemistry, and cell activation was determined based on cytokine and chemokine production. While the cell types investigated expressed TLR4 and CD44, no increased release of IL-1ß, IL-6, IL-8, IL-10, IL-12 or TNF-α was detected after HA stimulation. Similarly, HA did not enhance activation after priming cells with low doses of LPS or by forming complexes with LPS. Hence, this study does not support the common view of HA...Continue Reading

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Citations

Nov 24, 2019·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Olga KrupkovaKarin Wuertz-Kozak
May 19, 2020·Journal of Leukocyte Biology·Iwona NiemietzKelly L Brown
Jun 28, 2019·Inflammation·Mary K CowmanThorsten Kirsch

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Methods Mentioned

BETA
ELISA
FCS
Flow

Software Mentioned

Flow Cytometric Analysis Program ( FCAP ) Array Pro

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