Fragments of SLIT3 inhibit cellular migration

International Journal of Molecular Medicine
T SchubertAnja K Bosserhoff


The repellent factor family of Slit molecules has been described as having a repulsive function in the developing nervous system on growing axons expressing the Roundabout (Robo) receptors. Recent studies determined the effects of Slit molecules on the migratory and invasive potential of several types of tumor cells but also on synovial fibroblasts (SFs) derived from rheumatoid arthritis (RA) patients. To optimize a potential therapeutic application we aimed at generatingfragments of Slit3 showing the same functional ability as the full-length molecule but having the advantage of a smaller size. Recombinant Slit3 proteins were expressed and analyzed by western blotting. Their activity was defined by functional assays such as migration assays with RASF and melanoma cells. Recombinant Slit3 containing only leucine rich repeat domain 2 (D2), the domain important for Robo binding and the minimal functional unit D2 dNC were both able to inhibit migration of RASFs as effectively as Slit3 with all 4 repeats. Collectively, our data showed that the ability of Slit3 to reduce the migratory activity of synovial cells from patients with RA and melanoma cells can be mimicked by small protein fragments derived from Slit3. Slit3 fragments may...Continue Reading


Apr 10, 1999·Neuron·K T Nguyen Ba-CharvetA Chédotal
Feb 13, 2001·Neuron·S RajagopalanB J Dickson
May 5, 2005·Current Treatment Options in Oncology·Ehab Atallah, Lawrence Flaherty
May 20, 2006·Biochemical Society Transactions·E HohenesterJ A Howitt
Oct 13, 2006·Annual Review of Cell and Developmental Biology·Barry J Dickson, Giorgio F Gilestro
Nov 30, 2006·Biological Chemistry·Raphael StollAnja-Katrin Bosserhoff
Mar 21, 2007·Pigment Cell Research·Alexandra E DenkAnja K Bosserhoff
Sep 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Cecile MorlotAndrew A McCarthy
Oct 31, 2007·Journal of Neuro-oncology·Sonja MertschVolker Senner
Nov 3, 2007·The American Journal of Medicine·Vikas Majithia, Stephen A Geraci
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Nov 14, 2008·Molecular Biology of the Cell·Maria Cristina StellaPaolo M Comoglio
Dec 3, 2008·Neoplasia : an International Journal for Oncology Research·Hee Kyung KimHui Xu
Sep 29, 2009·Critical Reviews in Oncology/hematology·Roger MouawadDavid Khayat
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