Fragments of SLIT3 inhibit cellular migration

International Journal of Molecular Medicine
T SchubertAnja K Bosserhoff

Abstract

The repellent factor family of Slit molecules has been described as having a repulsive function in the developing nervous system on growing axons expressing the Roundabout (Robo) receptors. Recent studies determined the effects of Slit molecules on the migratory and invasive potential of several types of tumor cells but also on synovial fibroblasts (SFs) derived from rheumatoid arthritis (RA) patients. To optimize a potential therapeutic application we aimed at generatingfragments of Slit3 showing the same functional ability as the full-length molecule but having the advantage of a smaller size. Recombinant Slit3 proteins were expressed and analyzed by western blotting. Their activity was defined by functional assays such as migration assays with RASF and melanoma cells. Recombinant Slit3 containing only leucine rich repeat domain 2 (D2), the domain important for Robo binding and the minimal functional unit D2 dNC were both able to inhibit migration of RASFs as effectively as Slit3 with all 4 repeats. Collectively, our data showed that the ability of Slit3 to reduce the migratory activity of synovial cells from patients with RA and melanoma cells can be mimicked by small protein fragments derived from Slit3. Slit3 fragments may...Continue Reading

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Citations

Aug 21, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Alexander RudovMaria Cristina Albertini
Sep 24, 2016·Cancer Cell International·Anke RuedelAnja Katrin Bosserhoff
Mar 5, 2016·International Journal of Molecular Medicine·Yichun KongYing Chen
Apr 2, 2021·The Journal of Surgical Research·Kathleen MarulandaSean E McLean

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