Free amino acids hydroxyproline, lysine, and glycine promote differentiation of retinal pericytes to adipocytes: A protective role against proliferative diabetic retinopathy

Experimental Eye Research
S VidhyaS R Bharathidevi

Abstract

This study was conducted to estimate the aminoacid levels in the vitreous of patients with proliferative diabetic retinopathy, and to correlate it with the adiponectin levels. Secondly to test if these amino acids can alter or induce adiponectin levels and its related factors in retinal cells like pericyte as an in vitro model. All human studies were done as per declaration of Helsinki with institutional approval and after obtaining consent from participating individuals. The vitreous amino acids were estimated in PDR (Proliferative diabetic retinopathy) and MH (Macular Hole) as disease control using HPLC. Bovine retinal pericytes (BRP) were cultured in DMEM/F12 medium and treated with 0.5 mM of any one of the individual amino acids (proline, hydroxyproline, phenylalanine, alanine, serine, glycine, lysine, isoleucine or valine) along with 100 nM insulin for 14 days in high glucose (25 mM) condition. The mRNA expression profile of adipogenic markers (such as Pref1, APN, ZAG and PPARγ), angiogenic markers (VEGF, MMP-2 and MMP-9, TGF-β) and antioxidant markers (Nrf2 and UCP-2) were evaluated by qPCR. Adipogenesis was further confirmed by adipogenesis assay, secretion of adiponectin in medium and triglyceride accumulation by Oil re...Continue Reading

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Citations

Sep 25, 2019·Indian Journal of Ophthalmology·Subramaniam Rajesh Bharathi DeviNarayanasamy Angayarkanni
Dec 22, 2019·International Journal of Molecular Sciences·Nunzia CaporarelloGabriella Lupo
Dec 12, 2019·Frontiers in Cellular Neuroscience·Xiaojuan SuDezhi Mu
Oct 26, 2020·Diabetologia·Yohei TomitaYoshihiko Usui
Mar 21, 2021·Amino Acids·Kyeong Won LeeHyung-Soon Yim
Aug 5, 2021·Investigative Ophthalmology & Visual Science·Xiao-Wen HouChen-Wei Pan

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