Free flow and capillary isoelectric focusing of bacteria from the tomatoes plant tissues

Journal of Chromatography. a
Marie HorkáKarel Slais

Abstract

The means of the preconcentration and preseparation of selected species or pathovars of bacteria directly from the plant tissue suspension by free flow isoelectric focusing are introduced here. After the focusing, the resulting fraction of microorganisms, native or dynamically modified by the non-ionogenic tenside on the basis of pyrenebutanoate, was separated by capillary isoelectric focusing and/or cultivated and positively identified by gas chromatographic analysis of fatty acid methyl esters. Simultaneously, capillary isoelectric focusing with UV and fluorometric detection was used for the rapid estimation of unknown isoelectric points of the examined plant pathogenic species of genus Clavibacter, Xanthomonas and Pseudomonas prior to the preconcentration and preseparation. The microorganisms were of different origin, native and/or dynamically modified by the non-ionogenic tenside.

References

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Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·H CanutG Weber
Mar 9, 2006·Journal of Microbiological Methods·Peter DawyndtJean Swings
Apr 25, 2006·Journal of Microbiological Methods·L de LeónA Rodríguez
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Jun 13, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Marie HorkáKarel Slais
Aug 19, 2006·Molecular & Cellular Proteomics : MCP·Hans ZischkaMarius Ueffing

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Citations

Nov 10, 2012·Journal of Chromatography. a·Farid OukacineHervé Cottet
Jul 24, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jeff E PrestBernard J Treves Brown
Aug 14, 2012·Proteomics·Jiří ŠalplachtaMarie Horká
Jul 2, 2010·Electrophoresis·Gi Won ShinGyoo Yeol Jung
Mar 22, 2014·Journal of Separation Science·Cheng-Zhang YangXin-Qiao Jin
Apr 1, 2020·Journal of the American Chemical Society·M LukasK Meister
Jan 6, 2011·Analytical Chemistry·Farid OukacineHervé Cottet

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