Free-flow zone electrophoresis and isoelectric focusing using a microfabricated glass device with ion permeable membranes

Lab on a Chip
Dietrich KohlheyerRichard B M Schasfoort

Abstract

This paper describes a microfabricated free-flow electrophoresis device with integrated ion permeable membranes. In order to obtain continuous lanes of separated components an electrical field is applied perpendicular to the sample flow direction. This sample stream is sandwiched between two sheath flow streams, by hydrodynamic focusing. The separation chamber has two open side beds with inserted electrodes to allow ventilation of gas generated during electrolysis. To hydrodynamically isolate the separation compartment from the side electrodes, a photo-polymerizable monomer solution is exposed to UV light through a slit mask for in situ membrane formation. These so-called salt-bridges resist the pressure driven fluid, but allow ion transport to enable electrical connection. In earlier devices the same was achieved by using open side channel arrays. However, only a small fraction of the applied voltage was effectively utilized across the separation chamber during free-flow electrophoresis and free-flow isoelectric focusing. Furthermore, the spreading of the carrier ampholytes into the side channels resulted in a very restricted pH gradient inside the separation chamber. The chip presented here allows at least 10 times more effic...Continue Reading

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Citations

Mar 18, 2009·Analytical and Bioanalytical Chemistry·Ryan T Turgeon, Michael T Bowser
Sep 6, 2011·Analytical and Bioanalytical Chemistry·Stefan JezierskiDetlev Belder
Nov 13, 2007·Analytical Chemistry·Jacob W AlbrechtKlavs F Jensen
Mar 21, 2008·Analytical Chemistry·Bryan R Fonslow, Michael T Bowser
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Jul 21, 2011·Se pu = Chinese journal of chromatography·Pingli WangYukui Zhang
Dec 31, 2011·Biomicrofluidics·Dietmar Puchberger-EnenglGerald A Urban
Feb 28, 2014·Journal of Chromatography. a·Tadeusz H DzidoHartmut Frank
May 20, 2014·Analytical Chemistry·Fletcher J AgostinoSergey N Krylov
Nov 22, 2007·Lab on a Chip·Nicole Pamme
Aug 15, 2009·Lab on a Chip·Samuel Tia, Amy E Herr
Sep 19, 2015·Electrophoresis·Munseok ChoiSung-Jin Kim
Oct 19, 2010·Biosensors & Bioelectronics·Abdennour AbbasQuan Cheng
Oct 27, 2015·Analytical Chemistry·Matthew GeigerMichael T Bowser
Jan 19, 2007·Journal of Chromatography. a·Victoria N StoneBernard J Treves Brown
Mar 5, 2009·Electrophoresis·Greg J Sommer, Anson V Hatch
Dec 25, 2008·Electrophoresis·Kiyohito Shimura
Nov 30, 2007·Electrophoresis·Václav Kasicka
Nov 23, 2006·Electrophoresis·Jacob W Albrecht, Klavs F Jensen
Nov 6, 2008·Electrophoresis·Miroslava Stastna, Karel Slais
Feb 7, 2009·Electrophoresis·Hong Chen, Z Hugh Fan
Mar 26, 2009·Electrophoresis·Ryan T Turgeon, Michael T Bowser
Jan 31, 2008·Electrophoresis·Dietrich KohlheyerRichard B M Schasfoort
May 22, 2008·Electrophoresis·Karel Slais
May 28, 2010·Journal of Separation Science·Pingli WangYukui Zhang

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