PMID: 11901318Mar 20, 2002Paper

Free hemoglobin enhances tumor necrosis factor-alpha production in isolated human monocytes

The Journal of Trauma
Eddy H CarrilloHiram C Polk

Abstract

A systemic inflammatory response (SIR) is seen in approximately 75% of patients with complex blunt liver injuries treated nonoperatively. Many feel this response is caused by blood, bile, and necrotic tissue accumulation in the peritoneal cavity. Our current treatment for these patients is a delayed laparoscopic washout of the peritoneal cavity, resulting in a dramatic resolution of the SIR. Spectrophotometric analysis of the intraperitoneal fluid has confirmed the presence of high concentrations of free hemoglobin (Hb). We hypothesize that free Hb enhances the local peritoneal response by increasing tumor necrosis factor-alpha (TNF-alpha) production by monocytes, contributing to the local inflammatory response and SIR. Monocytes from five healthy volunteers were isolated and cultured in RPMI-1640 for 24 hours. Treatment groups included saline controls, lipopolysaccharide ([LPS], 10 ng/mL, from Escherichia coli), human Hb (25 microg/mL), and Hb + LPS. Supernatants were analyzed by enzyme-linked immunosorbent assay. Student's t test with Mann-Whitney posttest was used for statistical analysis with p < or = 0.05 considered significant. Free Hb significantly increased TNF-alpha production 915 +/- 223 pg/mL versus saline (p = 0.02)...Continue Reading

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Citations

Jan 16, 2004·Critical Care Medicine·Jacques Creteur, Jean-Louis Vincent
Jul 13, 2006·The Journal of Trauma·Mayur B PatelKenneth G Proctor
May 30, 2003·American Journal of Respiratory and Critical Care Medicine·Luis A AugustoRichard Chaby
Apr 28, 2009·Journal of Leukocyte Biology·Xueya LiangH Shaw Warren
Aug 28, 2007·Journal of Dental Research·C BodetD Grenier
Jan 31, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Zoltán SpolaricsEdwin A Deitch
May 15, 2009·Clinical and Experimental Immunology·J H M van der MeerC A Dinarello
Oct 22, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sharon MumbyGregory J Quinlan
Jul 7, 2020·Journal of Immunology Research·Vera P MouritsMihai G Netea
Apr 4, 2009·Critical Care Clinics·Jacques Creteur, Jean-Louis Vincent

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