Free radical mediated x-ray damage of model membranes

Biophysical Journal
A Cheng, M Caffrey

Abstract

The damaging effects of synchrotron-derived x rays on aqueous phospholipid dispersions have been evaluated. The effect of degree of lipid hydration, phospholipid chemical structure, mesophase identity, aqueous medium composition, and incident flux on the severity and progress of damage was quantified using time-resolved x-ray diffraction and chromatographic analysis of damage products. Electron spin resonance measurements of spin-trapped intermediates generated during irradiation suggest a free radical-mediated process. Surprisingly, radiation damage effects revealed by x-ray diffraction were imperceptible when the lamellar phases were prepared under water-stressed conditions, despite the fact that x-ray-induced chemical breakdown of the lipid occurred regardless of hydration level. Of the fully hydrated lipid systems studied, saturated diacyl-phosphatidylcholines were most sensitive to radiation damage compared to the ester- and ether-linked phosphatidylethanolamines and the ether-linked phosphatidylcholines. The inclusion of buffers or inorganic salts in the dispersing medium had only a minor effect in reducing damage development. A small inverse dose-rate effect was found when the x-ray beam intensity was changed 15-fold. Th...Continue Reading

Citations

Nov 10, 1998·Chemistry and Physics of Lipids·A ChengM Caffrey
Nov 6, 2007·The Review of Scientific Instruments·Stephen M DanauskasKa Yee C Lee
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·M WeikJ L Sussman
Aug 30, 2011·Journal of Structural Biology·Minna R KrejciDerk Joester
Mar 7, 1997·Biochemical Pharmacology·L A PeñaR Kolesnick
Apr 4, 2000·Structure·R B Ravelli, S M McSweeney
Sep 16, 2014·Physical Chemistry Chemical Physics : PCCP·Bekir SalgınMichael Rohwerder
May 19, 2001·Clinical Implant Dentistry and Related Research·E L LiljenstenP Thomsen
Jul 20, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Brendan DyettXuehua Zhang

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