Free radical scavenging, antimicrobial and immunomodulatory activities of Orthosiphon stamineus.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Mohammed A AlshawshNabil S Harmal

Abstract

Orthosiphon stamineus is considered an important traditional folk medicine. In this study ethanol and aqueous extracts of O. stamineus were evaluated in vitro for their antioxidant, antimicrobial as well as for their immunomodulatory properties on human peripheral blood mononuclear cells (PBMCs). The DPPH radical scavenging method was used for the determination of antioxidant activity, while the antibacterial efficacy was investigated by both disc diffusion method and Minimum Inhibitory Concentration (MIC) against four bacterial strains (Gram-positive and Gram-negative). Furthermore, the immunomodulatory potential of the extracts was investigated through the MTT assay. Aqueous extract of O. stamineus exhibited significant free radical scavenging activity with IC₅₀ 50 9.6 µg/mL, whereas the IC₅₀ for the ethanol extract was 21.4 µg/mL. The best antimicrobial activity was shown by the aqueous extract of O. stamineus against Staphylococcus aureus, with inhibition zone of 10.5 mm and MIC value 1.56 mg/mL. Moreover, the results observed from the MTT assay showed that both plant extracts stimulated the PBMCs proliferation in vitro in a concentration-dependent manner, but the aqueous extract has remarkable activity against PBMCs. These...Continue Reading

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Citations

Feb 13, 2014·Pakistan Journal of Biological Sciences : PJBS·Manjusha SaxenaYogesh Chaturvedi
Jun 3, 2014·Biological & Pharmaceutical Bulletin·Ronghui GuChunlin Long
Jun 22, 2017·Drug and Chemical Toxicology·Dhamraa Waleed Al-DualimiAmin Malik Shah Abdul Majid
Aug 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zili GuoYuanyuan Xie
Apr 24, 2020·Drug and Chemical Toxicology·Dhamraa W Al-DulaimiLoiy E Ahmed Hassan

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Methods Mentioned

BETA
Assay
density-gradient centrifugation
ELISA

Software Mentioned

SPSS

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