PMID: 9534884Apr 16, 1998Paper

Free radicals as potential mediators of metal allergy: effect of ascorbic acid on lymphocyte proliferation and IFN-gamma production in contact allergy to Ni2+ and Co2+

Acta Dermato-venereologica
L T Van Den BroekeA-T Karlberg

Abstract

A possible free radical mechanism in metal allergy was investigated in peripheral blood mononuclear cell (PBMC) cultures from 6 subjects, contact allergic to Ni2+ and Co2+, and 6 control individuals. Ni2+ and Co(2+)-mediated free radical generation was studied with electron spin resonance spectroscopy. The immune response was characterized by cellular [methyl-3H]thymidine uptake and interferon-gamma (IFN-gamma) production Ni2+ and Co2+ (10-50 microM) significantly increased lymphocyte proliferation and IFN-gamma production in PBMC cultures from contact allergic subjects in comparison with cultures from controls. Inhibition of Co(2+)-mediated free radical generation by ascorbic acid did not influence cellular [methyl-3H]thymidine uptake and IFN production. Detectable amounts of free radicals were not obtained with Ni2+. We therefore conclude that it is unlikely that free radicals are involved in contact allergy to Ni2+ and Co2+.

Citations

Jul 17, 1998·Contact Dermatitis·L T van den BroekeA T Karlberg
Jan 8, 2004·The Journal of Investigative Dermatology·Stefanie BruchhausenDetlef Becker
Feb 22, 2012·Archives of Biochemistry and Biophysics·Hubert ChangMartin Grootveld
Oct 24, 2017·Metallomics : Integrated Biometal Science·Vineet KumarDipak Dutta

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