Free-solution electrophoresis of DNA modified with drag-tags at both ends

Electrophoresis
R J MeagherA E Barron

Abstract

In end-labeled free-solution electrophoresis (ELFSE), DNA molecules are labeled with a frictional modifier or "drag-tag", allowing their size-based electrophoretic separation in free solution. Among the interesting observations from early work with dsDNA using streptavidin as a drag-tag was that the drag induced by including a streptavidin label at both ends was significantly more than double that from a single streptavidin (Heller, C. et al.., J. Chromatogr. A 1998, 806, 113-121). This finding was assumed to be in error, and subsequent work focused on experiments in which only a single drag-tag is appended to one end of the DNA molecule. Recent theoretical work (McCormick, L. C., Slater, G. W., Electrophoresis 2005, 26, 1659-1667) has examined the contribution of end-effects to the free-solution electrophoretic mobility of charged-uncharged polymer conjugates, reopening the question of enhanced drag from placing a drag-tag at both ends. In this study, this effect is investigated experimentally, using custom-synthesized ssDNA oligonucleotides allowing the attachment of drag-tags to one or both ends, as well as dsDNA PCR products generated with primers appropriate for the attachment of drag-tags at one or both ends. A range of s...Continue Reading

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Citations

Dec 25, 2010·Analytical Chemistry·Jennifer Coyne AlbrechtAnnelise E Barron
Jun 8, 2013·Analytical Chemistry·David W WegmanSergey N Krylov
May 20, 2008·Analytical Chemistry·Vratislav KostalEdgar A Arriaga
May 24, 2013·The Journal of Chemical Physics·Owen A Hickey, Christian Holm
May 12, 2007·The Journal of Chemical Physics·S NedelcuG W Slater
Jan 22, 2014·Journal of Chromatography. a·Anita RogacsJuan G Santiago
Jan 1, 2014·Electrophoresis·Mykyta V Chubynsky, Gary W Slater
May 3, 2007·Methods : a Companion to Methods in Enzymology·Fitsumbirhan ArayaRoger D Waigh
Oct 13, 2015·Analytical Chemistry·Stephen B IstivanJames W Schneider
Jan 15, 2015·Analytical Chemistry·Jiayin BaoSergey N Krylov

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