Free zinc concentration in bovine milk measured by analytical affinity chromatography with immobilized metallothionein

Biological Trace Element Research
P Zhang, J C Allen

Abstract

A new analytical affinity chromatography method was developed for measuring the free [Zn2+] concentration in bovine milk. The column was generated by immobilizing avidin and attaching biotinylated metallothionein (MT) on controlled-pore glass beads. Zinc bound to the MT column at physiological free [Zn2+] concentration and was dissociated again in an elution buffer of pH 2. The distributions of extrinsically added 65Zn and native zinc in different fractions of milk were virtually identical, validating the use of extrinsic labeling in studies of the free [Zn2+] concentration in milk. Extrinsically labeled whey fractions were mixed with standard solutions whose free [Zn2+] concentrations were calculated by computer model. 65Zn retained by the column provided an indication of free [Zn2+] concentration in the mixture, and by interpolation, in the original milk. The free [Zn2+] concentration measured by the affinity chromatography method in the milk of a group of six cows was 90.4 +/- 29.7 pM. This value is similar to estimates of free [Zn2+] concentrations in other biological fluids by entirely different methods. Measurement of free [Zn2+] may be helpful in understanding the physiology and biochemistry of zinc metabolism.

References

Apr 1, 1979·The American Journal of Clinical Nutrition·N W Solomons
Apr 1, 1979·The Journal of Pediatrics·K M HambidgeC Bender
Jun 1, 1992·Preparative Biochemistry·R D ComeauF A Liberatore
Aug 1, 1991·Biological Trace Element Research·S J Vaillancourt, J C Allen
May 1, 1989·The American Journal of Clinical Nutrition·C E CaseyK M Hambidge
May 1, 1989·The Journal of Dairy Research·H SinghP F Fox
Jun 1, 1987·The American Journal of Clinical Nutrition·J SchölmerichW Gerok
Mar 1, 1985·The Journal of Nutrition·B SandströmB Lönnerdal
Aug 1, 1973·The Biochemical Journal·N M Green, E J Toms
Feb 1, 1967·Journal of Dairy Science·S Parkash, R Jenness
Aug 1, 1981·The Journal of Nutrition·M P MenardR J Cousins
Jan 1, 1983·Annual Review of Nutrition·R M Forbes, J W Erdman
Jan 1, 1983·Annual Review of Nutrition·R A DiSilvestro, R J Cousins
Feb 1, 1983·The Biochemical Journal·P BlakeboroughM I Gurr
Nov 8, 1983·Biochimica Et Biophysica Acta·T C McGannT Richardson
Sep 1, 1983·The American Journal of Clinical Nutrition·B SandströmB Lönnerdal
Jul 1, 1980·The Journal of Nutrition·B C StarcherJ G Madaras
Jan 1, 1980·Journal of Inorganic Biochemistry·B LönnerdalL S Hurley
Aug 1, 1980·Journal of Inorganic Biochemistry·D S Norton, F W Heaton
Jul 1, 1994·Journal of Dairy Science·M C NevilleJ C Allen

❮ Previous
Next ❯

Citations

Mar 10, 2001·The British Journal of Nutrition·G E VegarudC Svenning
Oct 27, 2012·BMC Biotechnology·Mohammad Shohel Rana SiddikiIsamu Maeda

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.