Freeform micropatterning of living cells into cell culture medium using direct inkjet printing

Scientific Reports
Ju An ParkSungjune Jung

Abstract

Microfabrication methods have widely been used to control the local cellular environment on a micron scale. However, accurately mimicking the complexity of the in vivo tissue architecture while maintaining the freedom of form and design is still a challenge when co-culturing multiple types of cells on the same substrate. For the first time, we present a drop-on-demand inkjet printing method to directly pattern living cells into a cell-friendly liquid environment. High-resolution control of cell location is achieved by precisely optimizing printing parameters with high-speed imaging of cell jetting and impacting behaviors. We demonstrated the capabilities of the direct cell printing method by co-printing different cells into various designs, including complex gradient arrangements. Finally, we applied this technique to investigate the influence of the heterogeneity and geometry of the cell population on the infectivity of seasonal H1N1 influenza virus (PR8) by generating A549 and HeLa cells printed in checkboard patterns of different sizes in a medium-filled culture dish. Direct inkjet cell patterning can be a powerful and versatile tool for both fundamental biology and applied biotechnology.

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Citations

May 29, 2018·Small·Yanzhen ZhangGunther Wittstock
Aug 28, 2019·Scientific Reports·Hwa-Rim LeeSungjune Jung
Jul 9, 2020·American Journal of Physiology. Cell Physiology·Chiara ScognamiglioGianluca Cidonio
Feb 11, 2020·Advanced Functional Materials·Daeha JoungMichael C McAlpine
Jul 7, 2020·Biofabrication·Joseph P R DudmanKenneth W Dalgarno
May 1, 2018·Advanced Healthcare Materials·Sejeong YoonSungjune Jung
May 20, 2020·Biofabrication·Woong Hee YoonSungjune Jung
Oct 6, 2020·Macromolecular Bioscience·Jaideep AdhikariProsenjit Saha
Dec 22, 2020·Journal of Biomaterials Applications·Abhishek IndurkarPrajakta Dandekar
Nov 19, 2020·Advanced Materials·Pengfei Zhang, Adam R Abate
Jan 20, 2021·ACS Biomaterials Science & Engineering·Vittorio FerraraBruno Pignataro
Jan 15, 2019·Acta Biomaterialia·Ashley N LeberfingerIbrahim T Ozbolat
Jan 20, 2021·ACS Biomaterials Science & Engineering·Srimanta BaruiBrian Derby
Sep 10, 2020·Chemical Reviews·Xinda LiTao Xu

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Methods Mentioned

BETA
bioprinting
imaging techniques
chemical treatments
PCR
imaging technique

Software Mentioned

OriginPro
MATLAB

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