Freeze-all: enhanced outcomes with cryopreservation at the blastocyst stage versus pronuclear stage using slow-freeze techniques

Reproductive Biomedicine Online
Eric SurreyWilliam B Schoolcraft

Abstract

This retrospective cohort study compared outcomes from transfer of embryos cryopreserved at the pronuclear versus blastocyst stage following 'freeze-all' IVF cycles without fresh transfer for 87 consecutive IVF patients <40 years, who underwent cryopreservation of all viable embryos followed by at least one subsequent frozen embryo transfer (FET) between January 2003 and July 2007. Cryopreservation of all embryos from one oocyte retrieval was performed at either the pronuclear (1.5 mol/l propanediol and 0.1 mol/l sucrose) (group A) or blastocyst (10% glycerol) (group B) stage. Main outcome measures included survival, live birth and implantation rates. A total of 110 FET cycles were analysed. Live birth and implantation rates observed after the first FET were significantly higher (P=0.025 and P=0.002) in group B (67.7% and 40.8%) than in group A (41.1% and 21.5%) despite a higher survival rate in group A. After two FET cycles, 32.1% of group A had not conceived despite thaw of all available embryos, compared with 6.5% of group B. When freeze-all is necessary, blastocyst cryopreservation leads to higher implantation and live birth rates compared with pronuclear-stage cryopreservation despite lower survival rates. Prolonged embryo...Continue Reading

References

Jun 2, 2001·Human Reproduction·A TiitinenC Hyden-Granskog
Jan 10, 2002·Fertility and Sterility·Raja Z KarakiMohammed H Ibrahim
Mar 24, 2004·Fertility and Sterility·David K GardnerWilliam B Schoolcraft
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Citations

Apr 30, 2014·BioMed Research International·János KoncSándor Cseh
Dec 21, 2011·Archives of Gynecology and Obstetrics·Mário SousaAlberto Barros
Aug 11, 2011·Human Reproduction·Paul DevroeyChristophe Blockeel
Feb 5, 2016·Journal of Assisted Reproduction and Genetics·Ehab Darwish, Yasmin Magdi
Sep 6, 2011·Fertility and Sterility·Nursen MesutMustafa Bahceci
Aug 10, 2015·Fertility and Sterility·Bruce S ShapiroCynthia Hudson
Jun 4, 2014·Fertility and Sterility·Kai Mee WongSjoerd Repping
Feb 22, 2018·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Yamei XueSongying Zhang
Jul 27, 2021·Journal of Assisted Reproduction and Genetics·Margeaux OlivaLucky Sekhon
Jul 8, 2011·Current Opinion in Obstetrics & Gynecology

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