Freeze-drying of ATP entrapped in cationic, low lipid liposomes

Cryobiology
Véronique VincourtGilles Dumortier

Abstract

Concerning the instability of ATP liposomes formulated to easily diffuse through the liver (size approximately 100 nm), this work targets the key parameters that influence the freeze-drying of a preparation that combines cholesterol, DOTAP and phosphatidylcholine (either natural soybean or egg (SPC or EPC) or hydrogenated (HSPC)). After freeze-drying blank liposomes, size increased significantly when initial lipid concentration was lowered from 20 to 5mM (p=0.0018). With low lipid concentration preparation (5mM), SPC limited size increase (SI) more efficiently compared to EPC or HSPC. With SPC and EPC, sucrose showed better size results compared to trehalose (Lyoprotectant/Lipid ratio (w/w) avoiding any SI: approximately 5 and approximately 10 (for SPC), approximately 10 and approximately 15 (for EPC), for sucrose and trehalose, respectively), but the opposite was evidenced with HSPC liposomes where a Trehalose/Lipid ratio of 25 barely prevented SI. In addition, slow versus quick cooling rate led to limiting SI for HSPC liposomes (p=0.0035). With sucrose or trehalose at both Lyoprotectant/Lipid ratios ensuring size stabilisation (10:1 and 15:1, respectively), ATP leakage ranged between 38.8+/-7.9% and 58.2+/-1.4%. In conclusion...Continue Reading

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Citations

May 10, 2013·Expert Opinion on Drug Delivery·Khaled Mohamed HosnyRana Tariq Al-Abdali
Apr 28, 2012·International Journal of Pharmaceutics·Anumita ChaudhuryGigi N C Chiu
Nov 29, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Alena WieberJörg Kreuter
Jun 3, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Guendalina ZuccariMirco Ponzoni
Aug 30, 2018·Pharmaceutics·Silvia FranzéFrancesco Cilurzo
Feb 25, 2019·Advanced Drug Delivery Reviews·Marie RouquettePatrick Couvreur

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