PMID: 7031067Nov 1, 1981Paper

Freeze-fracture cytochemistry: localization of wheat-germ agglutinin and concanavalin A binding sites on freeze-fractured pancreatic cells

The Journal of Cell Biology
P Pinto da SilvaB Kachar

Abstract

The combined application of thin-section and critical-point-drying "fracture-label" is used to determine the pattern of distribution and partition of wheat-germ agglutinin and concanavalin A binding sites on the membrane faces of freeze-fractured exocrine and endocrine rat pancreatic cells. Whereas the exoplasmic face of plasma membrane is preferentially labeled by both lectins, the endoplasmic reticulum and nuclear envelope are strongly and uniformly labeled by concanavalin A but not by wheat-germ agglutinin. The results support current views in the glycosylation of membrane proteins and do not support the backflow of sialidated glycoproteins to the endoplasmic reticulum.

References

Apr 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Horisberger, J Rosset
Oct 24, 1977·Biochemical and Biophysical Research Communications·I Virtanen
May 21, 1976·Biochimica Et Biophysica Acta·A Bergman, G Dallner
Jun 1, 1974·FEBS Letters·A Monneron, D Segretain
Jun 1, 1970·The Journal of Cell Biology·P Pinto da Silva, D Branton
May 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·D Branton
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P Pinto da SilvaN Dwyer
Aug 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P P da SilvaN Dwyer
Jan 1, 1980·The Journal of Cell Biology·R BretzG E Palade

❮ Previous
Next ❯

Citations

Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M R Torrisi, P P Da Silva
Feb 1, 1985·The Journal of Cell Biology·A P Aguas, P Pinto da Silva
Jan 1, 1984·The Journal of Cell Biology·M R Torrisi, P Pinto da Silva
Sep 1, 1984·The Journal of Cell Biology·P Pinto da Silva, F W Kan
Jan 1, 1988·Critical Reviews in Clinical Laboratory Sciences·A Raedler, S Schreiber
Oct 24, 2008·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·P Taupin
May 1, 1993·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·J KriegsmannR Bräuer
Dec 1, 1983·Journal of Cellular Physiology·S E Myrdal, R L DeHaan
Jan 1, 1989·Archives of Biochemistry and Biophysics·D Y ZhaoN S Dhalla
Jul 15, 1988·Biochemical and Biophysical Research Communications·N MakinoN S Dhalla
Sep 23, 1997·Biochemical and Biophysical Research Communications·T LuckcuckJ H Walker
Jun 27, 2006·Cell Biology International·Pilar Cabezas, Cristina Risco
Apr 1, 1988·Journal of Electron Microscopy Technique·F W Kan, A Nanci
Nov 1, 1989·Journal of Electron Microscopy Technique·N J Severs
Jan 1, 1992·Journal of Virology·M R TorrisiG Campadelli-Fiume
Oct 5, 1987·Biochimica Et Biophysica Acta·J Roth
Jul 1, 1990·Journal of Bacteriology·R L Oropeza-WekerleW Goebel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.