PMID: 39080Apr 1, 1979

Freeze-fracture studies of frog neuromuscular junctions during intense release of neurotransmitter. II. Effects of electrical stimulation and high potassium

The Journal of Cell Biology
B CeccarelliW P Hurlbut

Abstract

Frog cutaneous pectoris nerve muscle preparations were studied by the freeze-fracture technique under the following conditions: (a) during repetitive indirect stimulation for 20 min, 10/s; (b) during recovery from this stimulation; and (c) during treatment with 20 mM K+. Indirect stimulation causes numerous dimples or protuberances to appear on the presynaptic membrane of nerve terminal, and most are located near the active zones. Deep infoldings of the axolemma often develop between the active zones. Neither the number nor the distribution of dimples, protuberances, of infoldings changes markedly during the first minute of recovery. The number of dimples, protuberances, and infoldings is greatly reduced after 10 min of recovery. Since endocytosis proceeds vigorously during the recovery periods, we conclude that endocytosis occurs mostly at the active zones, close to the sites of exocytosis. 20 mM K+ also causes many dimples or protuberances to appear on the axolemma of the nerve terminal but they are distributed almost uniformly along the presynaptic membrane. Experiments with horseradish peroxidase (HRP) show that recycling of synaptic vesicles occurs in 20 mM K+. This recycling is not accompanied by changes in the number of ...Continue Reading

References

May 1, 1996·Journal of Neuroscience Research·C Colasante, M Pécot-Dechavassine
Nov 9, 2000·Cell Biology International·M E KriebelO M Brown
Apr 1, 1985·Journal of Neurocytology·A Triller, H Korn
Apr 1, 1986·Journal of Neurocytology·J PearceR R Shivers
Sep 21, 2012·Molecular Neurobiology·Raquel CanoLucía Tabares
Jan 1, 1996·Neurochemistry International·R FesceJ Meldolesi
Nov 1, 1992·Progress in Neurobiology·T Gotow
Jan 1, 1979·Neuroscience·H Zimmermann
Feb 1, 1988·Neuroscience·F ValtortaB Ceccarelli
Jan 1, 1989·Neuroscience·F González-Aguilar, R H Alzola
Jan 1, 1990·Neuroscience·F ValtortaB Ceccarelli
Aug 8, 2001·Trends in Cell Biology·F ValtortaR Fesce
Oct 10, 1998·The European Journal of Neuroscience·D A RaciborskaM P Charlton
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A PerreletL Orgi
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K M BuckleyR B Kelly
Oct 1, 1980·The Journal of Cell Biology·B Ceccarelli, W P Hurlbut
Mar 1, 1981·The Journal of Cell Biology·J E Heuser, T S Reese
Feb 1, 1984·The Journal of Cell Biology·T M Miller, J E Heuser
May 1, 1984·The Journal of Cell Biology·C P Ko

Related Concepts

Rana pipiens
Salientia
Potassium
Synapses
Neurohormones
Freeze Fracturing
Exocytosis
Nerve-Muscle Preparation
Electric Stimulation Technique

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.