PMID: 6411740Sep 1, 1983Paper

Freeze-fracture studies of photoreceptor membranes: new observations bearing upon the distribution of cholesterol

The Journal of Cell Biology
L D Andrews, A I Cohen

Abstract

We performed electron microscopy of replicas from freeze-fractured retinas exposed during or after fixation to the cholesterol-binding antibiotic, filipin. We observed characteristic filipin-induced perturbations throughout the disk and plasma membranes of retinal rod outer segments of various species. It is evident that a prolonged exposure to filipin in fixative enhances rather than reduces presumptive cholesterol detection in the vertebrate photoreceptor cell. In agreement with the pattern seen in our previous study (Andrews, L.D., and A. I. Cohen, 1979, J. Cell Biol., 81:215-228), filipin-binding in membranes exhibiting particle-free patches seemed largely confined to these patches. Favorably fractured photoreceptors exhibited marked filipin-binding in apical inner segment plasma membrane topologically confluent with and proximate to the outer segment plasma membrane, which was comparatively free of filipin binding. A possible boundary between these differing membrane domains was suggested in a number of replicas exhibiting lower filipin binding to the apical plasma membrane of the inner segment in the area surrounding the cilium. This area contains a structure (Andrews, L. D., 1982, Freeze-fracture studies of vertebrate ph...Continue Reading

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Oct 20, 1977·Nature·G L FainH M Gerschenfeld
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Sep 1, 1976·Experimental Eye Research·J M CorlessJ D Robertson
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Citations

Jul 26, 2008·The Journal of Clinical Investigation·Mark E Kleinman, Jayakrishna Ambati
Sep 22, 2010·The Journal of Cell Biology·Ching-Hwa Sung, Jen-Zen Chuang
Dec 10, 1984·The Journal of Comparative Neurology·N G Cooper, B J McLaughlin
Oct 4, 2006·Free Radical Biology & Medicine·Anna Wisniewska, Witold K Subczynski
Apr 9, 2013·Progress in Retinal and Eye Research·Jillian N PearringVadim Y Arshavsky
Apr 18, 1997·The Journal of Biological Chemistry·E B Rodriguez de TurcoD S Papermaster

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