PMID: 9541215Apr 16, 1998Paper

Freeze-fracture studies of the distal plasma membrane of rat odontoblasts during their differentiation and polarisation

European Journal of Oral Sciences
V E Arana-Chavez, E Katchburian

Abstract

We have examined freeze-fracture replicas of maxillary first molar tooth germs of newborn rats at early stages of dentinogenesis to study the development of tight junctions in the distal plasma membrane of differentiating odontoblasts. In addition, freeze-fracture was combined with filipin to observe the distribution of cholesterol on the distal plasma membrane of odontoblasts during differentiation. Only gap junctions were present in early differentiating odontoblasts. The distal plasma membrane exhibited low cholesterol content, which might indicate high fluidity. With the beginning of mineral deposition in matrix-vesicles, the first signs of tight junction formation were observed. Further development revealed increasingly complex focal tight junctions. In later stages, when mineralisation is observed progressing to the fibrillar and non-fibrillar constituents of the matrix, well developed focal tight junctions were detected. Concomitantly, cholesterol in distal portions of the odontoblast plasma membrane increased, indicating, probably, a higher rigidity. Thus, a distal plasma membrane domain is established, odontoblasts become fully differentiated, and partial compartmentalisation of matrix occurs. At this stage, odontoblas...Continue Reading

Citations

Mar 31, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Silvia M A João, Victor E Arana-Chavez
Nov 22, 2012·European Journal of Oral Sciences·So-Young YangSun-Hun Kim
May 11, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·A J Smith, H Lesot
Jan 28, 2003·Histochemistry and Cell Biology·Silvia M A João, Victor E Arana-Chavez

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