Freeze-Thaw Cycling Induced Isotropic-Nematic Coexistence of Amyloid Fibrils Suspensions

Langmuir : the ACS Journal of Surfaces and Colloids
Jianguo ZhaoRaffaele Mezzenga

Abstract

Amyloid fibrils are charged semiflexible assemblies with very large aspect ratio (length to diameter, L/D). Because of this large aspect ratio, the isotropic (I) and nematic (N) phase coexistence expected from the first-order thermodynamic nature of the I-N phase transition, as predicted from the Onsager's theory, is vanishingly small and, in practice, challenging to experimentally observe. In this study we present a remarkable widening of the I + N biphasic region in β-lactoglobulin fibrils suspension via freeze-thaw (F-T) cycling. The demixing behavior can be induced and controlled by a slow growth of propagation front of the ice crystals, which grow by excluding the amyloid fibrils from the crystal phase and thus concentrating them in the liquid phase. The growth of the ice crystals is accompanied by the formation of concentrated and elongated tactoid-like structure in the liquid phase. During the subsequent thawing cycling, at large tactoid domains, the mismatch in density caused by the presence of amyloid fibrils is sufficient to generate a sedimentation of the N phase at the bottom of the vial, coexisting with an I phase on the top. We reason why, despite the remarkable stability of the coexisting I and N phases observed ...Continue Reading

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Citations

Oct 8, 2016·Lab on a Chip·Viviane Lutz-BuenoMarianne Liebi
Dec 13, 2018·Chemical Communications : Chem Comm·Xiangsheng HanChaoxu Li
Mar 21, 2019·Small·Jianguo ZhaoBen Bin Xu
May 15, 2018·Physical Review Letters·Yiping CaoRaffaele Mezzenga
Apr 30, 2017·Scientific Reports·Daniele VigoloRaffaele Mezzenga
Aug 31, 2017·ACS Omega·Saranyoo SornkamnerdTatsuo Kaneko
Nov 1, 2018·Nano Letters·Charlotte H ChenSamuel I Stupp
Aug 18, 2017·Biomacromolecules·Gustav NyströmRaffaele Mezzenga

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