Frequencies of X-ray induced pericentric inversions and centric rings in human blood lymphocytes detected by FISH using chromosome arm specific DNA libraries

Mutation Research
A T NatarajanA S Balajee

Abstract

Frequencies of intra-chromosomal exchanges (pericentric inversions and centric rings) and inter-chromosomal exchanges (dicentrics and translocations) in X-irradiated (2.5 Gy) human lymphocytes have been estimated. To detect these events we employed FISH (fluorescence in situ hybridization) technique and arm specific painting probes for chromosomes #1 and #3. The ratio between centric rings and pericentric inversions was found to be about 1. For intra-changes to inter-changes, the ratio (F) was between 6 and 9. Based on the total number of colour junctions involving chromosomes #1 and #3 it was found that exchanges between the arms of the same chromosome occur about 8.7 times more than inter-chromosomal exchanges calculated on the basis of the DNA content of the chromosomes and random induction of aberrations in the total genome. Chromosomal organization in interphase nucleus appears to promote the formation of more intra-changes than inter-changes following X-irradiation, most probably due to close proximity of the two arms of a chromosome.

References

Apr 1, 1992·Genes, Chromosomes & Cancer·H TeleniusB A Ponder
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·N E Morton
Jul 1, 1973·Mutation Research·J R Savage, D G Papworth
Feb 1, 1994·Mutation Research·J R Savage, P J Simpson
Jan 1, 1995·Cytogenetics and Cell Genetics·J D TuckerC Shasserre
Jan 1, 1996·Nature Genetics·X Y GuanJ Trent
May 27, 2004·Cytogenetic and Genome Research·J R K Savage

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Citations

Dec 4, 2003·Mutation Research·A T Natarajan, J J W A Boei
Jul 11, 2002·Mutation Research·A T Natarajan
Jul 11, 2002·Mutation Research·J J W A BoeiA T Natarajan
Nov 25, 2003·International Journal of Radiation Biology·A GüerciA Seoane
Apr 11, 2012·Mutation Research. Reviews in Mutation Research·Adayapalam T Natarajan
Sep 13, 2016·Nature Communications·Sam BehjatiPeter J Campbell
Feb 2, 2006·International Journal of Radiation Biology·Sylwester SommerAndrzej Wojcik
Nov 6, 2018·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Adayabalam S Balajee, M Prakash Hande

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