Frequency analysis of CD4+CD8+ T cells cloned with IL-4

Cellular Immunology
S A BrodD A Hafler

Abstract

The coexpression of both CD4 and CD8 molecules on T cells occurs in the peripheral blood at a low frequency and can be generated transiently on CD4+ peripheral blood T cells by treatment with lectin which induces CD8 biosynthesis and cell surface expression. We have cloned T cells in a nonselective fashion from normal subjects in the presence of either IL-2, rIL-4 and IL-2, or rIL-4 and have examined the phenotypic expression of CD4 and CD8. The addition of excess rIL-4 increased the expression of CD8 on the surface of CD4+ T cell clones but did not increase CD4 expression on CD8+ T cell clones. There were three patterns of CD4 and CD8 expression observed: high density CD8 with no CD4 expression; high density CD4 with low CD8 expression; or high density CD4 with higher cell surface CD8 expression which was regulated by the presence of rIL-4. CD4+ T cell clones originally cultured in IL-2 and rIL-4 and subsequently grown in IL-2 alone exhibited decreased expression of the CD8 molecule. The increased expression of CD8 did not correlate with NK activity or lectin-dependent cytotoxicity in an antigen independent system. In addition, rIL-4 alone or in combination with IL-2 appeared to accelerate the growth curve of T cell clones as ...Continue Reading

References

Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J PèneJ Banchereau
Jul 23, 1987·Nature·B P SleckmanS J Burakoff
Apr 1, 1988·The Journal of Experimental Medicine·D A HaflerH L Weiner
Jun 1, 1983·Clinical Immunology and Immunopathology·J T KurnickC P Leary
Jan 1, 1983·Immunological Reviews·S L Swain
Jan 1, 1984·Annual Review of Immunology·K A Smith
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E L ReinherzS F Schlossman
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S C MeuerE L Reinherz

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Citations

Jun 24, 2005·Cytometry. Part B, Clinical Cytometry·Cherry EdwardsDavid Barnett
Oct 1, 1994·Veterinary Immunology and Immunopathology·M D PescovitzF A Zuckermann
Jun 15, 1996·Veterinary Immunology and Immunopathology·D G BreedA N Vermeulen
Apr 28, 2004·Autoimmunity Reviews·Yann Parel, Carlo Chizzolini
Jun 8, 2001·Veterinary Immunology and Immunopathology·J HernándezE Zenteno
Dec 30, 1999·Veterinary Immunology and Immunopathology·F A Zuckermann
Dec 30, 1991·Annals of the New York Academy of Sciences·D A HaflerH L Weiner
Nov 18, 2015·Virology Journal·Zhijun LiEn-Min Zhou
Jul 1, 1991·European Journal of Immunology·G LeclercqJ Plum
Oct 1, 1990·European Journal of Immunology·S A BrodD A Hafler

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