Frequency and therapy monitoring of canine Babesia spp. infection by high-resolution melting curve quantitative FRET-PCR

Veterinary Parasitology
Chengming WangBernhard Kaltenboeck

Abstract

Babesia gibsoni and Babesia canis are the etiological agents of canine babesiosis, a protozoal hemolytic disease with global significance. Canine babesiosis has been diagnosed by microscopic identification of intra-erythrocytic trophozoites in blood smear, and by serological testing. Here we developed a quantitative fluorescence resonance energy transfer (FRET)-PCR that amplifies a fragment of the Babesia spp. 18S rRNA gene with high sensitivity and specificity. Melting curve analysis differentiates B. gibsoni, B. canis canis/B. canis vogeli, and B. canis rossi by the disassociation temperature of the fluorescent probes. Babesia gibsoni infection was detected in 8 of 48 canine breeds (17%) and 24 of a total of 235 specimens (10.2%) submitted from 22 states of the continental United States of America. A potential blood donor was positive for B. canis vogeli infection. In Hong Kong (China), B. gibsoni infection was detected in 30 of 64 specimens (46.9%) from 15 of the 24 breeds (63%). While the frequency of canine babesiosis did not associate with seasonal change in Hong Kong, positivity in the USA for Babesia spp. infection was higher in Spring and Summer than in Autumn and Winter. The data suggest that environmental factors ass...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1989·The Veterinary Quarterly·G UilenbergA A Spanjer
Feb 7, 2002·Journal of the American Veterinary Medical Association·Douglass K MacintirePatricia A Conrad
Apr 11, 2003·Journal of the American Veterinary Medical Association·Julie R StegemanEdward B Breitschwerdt
Aug 13, 2003·The Veterinary Clinics of North America. Small Animal Practice·A Lindsay Boozer, Douglass K Macintire
Sep 24, 2004·Veterinary Parasitology·A J BirkenheuerE B Breitschwerdt
Feb 8, 2006·The Pediatric Infectious Disease Journal·Leanne M FoxOfer Levy
Nov 28, 2006·Journal of Biochemical and Biophysical Methods·Chengming WangBernhard Kaltenboeck
Jul 19, 2007·Journal of Vector Ecology : Journal of the Society for Vector Ecology·A ZákovskáH Martiníková
Jul 8, 2008·Veterinary Parasitology·Luís CardosoGad Baneth
Sep 9, 2008·Veterinary Parasitology·Yihang LiBernhard Kaltenboeck
Sep 13, 2008·Veterinary Parasitology·Diego Fernando EirasLeonhard Schnittger
Nov 15, 2008·Veterinary Parasitology·Kursat AltayMunir Aktas
May 12, 2009·Parasites & Vectors·Peter J Irwin
Apr 19, 2015·Parasites & Vectors·Gary Kwok Cheong LeePeter John Irwin

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Citations

May 17, 2011·Veterinary Parasitology·Laia Solano-Gallego, Gad Baneth
Oct 12, 2010·The Veterinary Clinics of North America. Small Animal Practice·Peter J Irwin
Oct 3, 2015·Parasites & Vectors·Da XuChengming Wang
Mar 18, 2015·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Amornmas KongkliengWanchai Maleewong
Sep 26, 2013·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Clayton S DuttonEsteban Soto
Mar 28, 2012·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Kristen A PhairLisa M Pohlman

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