PMID: 9185680May 23, 1997Paper

Frequency-dependent expression of diaphorase staining and nNOS-immunoreactivity in rat dorsal horn neurones following C-fibre stimulation

Neuroscience Letters
U HoheiselS Mense

Abstract

Recent evidence demonstrated that lesion-induced central nervous system changes are at least partly mediated by the action of the gaseous transmitter nitric oxide (NO). We investigated the hypothesis that the frequency of peripheral C-fibre stimulation determines the number of neurones in the dorsal horn that can be visualised immunohistochemically with antibodies to NO synthase (NOS) or using the NADPH-dependent diaphorase (NDP) reaction. C-fibre stimulation of the sciatic nerve at a frequency of 0.01 Hz was followed by a significant increase in NDP-cell number in the spinal segment L3, whereas 0.1 and 1 Hz stimulation resulted in a significant decrease. Neuronal NOS (nNOS)-immunoreactivity was significantly influenced only by 1 Hz stimulation and only on the ipsilateral side in L3. Here, the number of nNOS-immunoreactive (ir) neurones decreased significantly in the superficial dorsal horn. The results show that the system of NDP-neurones is capable of displaying a bidirectional response depending on the frequency of C-fibre input.

References

Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B T HopeS R Vincent
Jun 1, 1996·Trends in Pharmacological Sciences·J J ReidP D Marley

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