Frequency doubling in the cyanobacterial circadian clock

Molecular Systems Biology
Bruno M C MartinsJames C W Locke

Abstract

Organisms use circadian clocks to generate 24-h rhythms in gene expression. However, the clock can interact with other pathways to generate shorter period oscillations. It remains unclear how these different frequencies are generated. Here, we examine this problem by studying the coupling of the clock to the alternative sigma factor sigC in the cyanobacterium Synechococcus elongatus Using single-cell microscopy, we find that psbAI, a key photosynthesis gene regulated by both sigC and the clock, is activated with two peaks of gene expression every circadian cycle under constant low light. This two-peak oscillation is dependent on sigC, without which psbAI rhythms revert to one oscillatory peak per day. We also observe two circadian peaks of elongation rate, which are dependent on sigC, suggesting a role for the frequency doubling in modulating growth. We propose that the two-peak rhythm in psbAI expression is generated by an incoherent feedforward loop between the clock, sigC and psbAI Modelling and experiments suggest that this could be a general network motif to allow frequency doubling of outputs.

References

Nov 25, 2017·Scientific Reports·Johan H van HeerdenFrank J Bruggeman
Sep 20, 2019·International Journal of Molecular Sciences·Nikolai GenovGianluigi Mazzoccoli
Nov 10, 2018·Proceedings of the National Academy of Sciences of the United States of America·Bruno M C MartinsJames C W Locke
Jul 1, 2017·Applied Microbiology and Biotechnology·Lucía Feijoo-SiotaTomás G Villa

Citations

Jun 15, 1995·Genes & Development·Y LiuTakao Kondo
Apr 1, 1995·Journal of Bacteriology·Susan S Golden
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A K ClarkeG Oquist
Jun 3, 1998·Applied and Environmental Microbiology·J B AndersenS Molin
Oct 12, 2000·Biochemical and Biophysical Research Communications·Y G ChenJ H Wu
Jun 12, 2002·Journal of Bacteriology·Usha NairSusan S Golden
Oct 7, 2003·Annual Review of Microbiology·Tanja M Gruber, Carol A Gross
Oct 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·S Mangan, Uri Alon
Apr 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Subhayu BasuRon Weiss
Jul 2, 2004·Nature·Irina MihalcescuStanislas Leibler
Aug 25, 2004·Current Biology : CB·Mark A WoelfleCarl Hirschie Johnson
Jun 14, 2005·Nature Reviews. Genetics·Deborah Bell-PedersenMark J Zoran
Apr 6, 2006·The Plant Cell·C Robertson McClung
Oct 30, 2007·Nature Structural & Molecular Biology·Hiroshi ItoTakao Kondo
Dec 22, 2007·Nature·Jeffrey R ChabotAlexander van Oudenaarden
Sep 13, 2008·Current Biology : CB·David C Wilcockson, Lin Zhang
Nov 26, 2008·Nature Genetics·Mary J DunlopMichael B Elowitz
Apr 4, 2009·PLoS Genetics·Michael E HughesJohn B Hogenesch
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi ItoHideo Iwasaki
Sep 4, 2009·Journal of Chromatography. a·Daifeng JiangWilliam E Haskins
Oct 20, 2009·Gene Regulation and Systems Biology·Sousuke Imamura, Munehiko Asayama
Dec 3, 2009·Molecular Systems Biology·Harish ShankaranH Steven Wiley
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Vikram VijayanErin K O'Shea
Sep 3, 2010·Annual Review of Genetics·Colleen J Doherty, Steve A Kay
Feb 22, 2011·Annual Review of Biophysics·Carl Hirschie JohnsonMartin Egli
May 11, 2011·Molecular Systems Biology·Eric BatchelorGalit Lahav
Oct 8, 2011·Science·James C W LockeMichael B Elowitz
Feb 1, 2012·PloS One·Luca Cerone, Zoltán Neufeld
Apr 10, 2012·Annual Review of Neuroscience·Jennifer A MohawkJoseph S Takahashi
Nov 13, 2012·PloS One·Anja KorenčičHanspeter Herzel
Apr 16, 2013·Cell Reports·Pål O Westermark, Hanspeter Herzel
Aug 16, 2014·Current Biology : CB·Gopal K PattanayakMichael J Rust
Nov 5, 2014·Photosynthesis Research·Rayka YokooDavid F Savage
Nov 8, 2014·Biochemistry·Zeenat B Noordally, Andrew J Millar

Related Concepts

Single-Cell Analysis
Synechococcus
Molecular Imaging
Gene Expression Regulation, Bacterial
Biochemical Pathway
Bacterial Proteins
Circadian Clocks
Time-Lapse Imaging
Cyanobacterium (antigen)
Gene Expression

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.