Frequency of Antibiotic-Producing Pseudomonas spp. in Natural Environments.

Applied and Environmental Microbiology
J M RaaijmakersLinda Thomashow

Abstract

The antibiotics phenazine-1-carboxylic acid (PCA) and 2,4-diacetylphloroglucinol (Phl) are major determinants of biological control of soilborne plant pathogens by various strains of fluorescent Pseudomonas spp. In this study, we described primers and probes that enable specific and efficient detection of a wide variety of fluorescent Pseudomonas strains that produce various phenazine antibiotics or Phl. PCR analysis and Southern hybridization demonstrated that specific genes within the biosynthetic loci for Phl and PCA are conserved among various Pseudomonas strains of worldwide origin. The frequency of Phl- and PCA-producing fluorescent pseudomonads was determined on roots of wheat grown in three soils suppressive to take-all disease of wheat and four soils conducive to take-all by colony hybridization followed by PCR. Phenazine-producing strains were not detected on roots from any of the soils. However, Phl-producing fluorescent pseudomonads were isolated from all three take-all-suppressive soils at densities ranging from approximately 5 x 10(sup5) to 2 x 10(sup6) CFU per g of root. In the complementary conducive soils, Phl-producing pseudomonads were not detected or were detected at densities at least 40-fold lower than tho...Continue Reading

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Citations

Jul 27, 2002·Annual Review of Microbiology·Angela D Kent, Eric W Triplett
Jul 31, 2002·Annual Review of Phytopathology·David M WellerLinda S Thomashow
Dec 3, 2003·Environmental Microbiology·J T de SouzaJ M Raaijmakers
Mar 17, 2006·Canadian Journal of Microbiology·Palaniyandi VelusamyLinda Thomashow
Nov 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·R James Cook
Mar 31, 2007·Applied Microbiology and Biotechnology·Christel SchmeisserWolfgang R Streit
Jun 15, 2007·Environmental Microbiology·Agnès RobinPhilippe Lemanceau
Mar 19, 2008·Microbial Ecology·Popavath Ravindra NaikNatarajan Sakthivel
Feb 3, 2009·Applied and Environmental Microbiology·Jennifer A MoynihanE Fidelma Boyd
Nov 16, 2010·Canadian Journal of Microbiology·Geoffrey G Bradley, Zamir K Punja
Mar 3, 2011·FEMS Microbiology Reviews·Mark W SilbyRobert W Jackson
Feb 20, 2013·Marine Biotechnology·Sumihiro KoyamaTaishi Tsubouchi
Apr 28, 2001·Journal of Experimental Botany·J M Whipps
Nov 9, 2002·Molecular Plant-microbe Interactions : MPMI·Henk-jan SchoonbeekMaarten A De Waard
Jan 20, 2007·FEMS Microbiology Ecology·Wietse de BoerJohannes A van Veen
Feb 22, 2005·The New Phytologist·Pascale Frey-KlettJean Garbaye
Jun 12, 2009·Critical Reviews in Microbiology·A S Ninawe, Joseph Selvin

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