PMID: 9175916Jun 1, 1997Paper

Frequency of clonal dominance in the specific antibody response to DNP-HSA in CBA and C57BL mice reflects their susceptibility to age-associated development of monoclonal gammopathies

Clinical Immunology and Immunopathology
C van ArkelJ Radl

Abstract

The effects of age, genetic background, and neonatal thymectomy on the levels and the heterogeneity of the specific antibody response were investigated in an experimental mouse model. Both intact and neonatally thymectomized (NTx) C57BL/KaLwRij (C57BL) and CBA/BrARij (CBA) mice were immunized at the age of 3 ("young") or 22 months ("old"). Highly sensitive antigen-specific immunoblotting techniques (ABL), in combination with agar-electrophoresis and isoelectric focusing (IEF), were used to investigate total specific antibody levels, the number of responding antigen-specific clonotypes, and the dominance of responding B cell clones in the antibody response against dinitrophenylated human serum albumin. After immunization, the specific antibody levels progressively increased in all experimental groups with the exception of old C57BL mice. All mice responded with a specific polyclonal heterogeneous response. In addition, some mice showed a clonal dominance of antibody-producing cells, as is reflected in the appearance of distinct homogeneous antibody components (H-Ab) in the sera. This clonal dominance was scarce in CBA mice but frequent in C57BL mice. Age at time of immunization and NTx had little if any additive effect on the in...Continue Reading

References

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Citations

Nov 9, 2000·Immunology Today·A Globerson, R B Effros
Oct 17, 2001·Experimental Gerontology·P Huijbers, D L Knook
Oct 27, 2004·Experimental Gerontology·Giuseppina Colonna-RomanoCalogero Caruso

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